Measurement of the differences in electrophoretic mobilities of individual molecules of E. coli beta-galactosidase provides insight into structural differences which underlie enzyme microheterogeneity.

Nichols ER; Craig DB

首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Measurement of the differences in electrophoretic mobilities of individual molecules of E. coli beta-galactosidase provides insight into structural differences which underlie enzyme microheterogeneity.

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Measurement of the differences in electrophoretic mobilities of individual molecules of E. coli beta-galactosidase provides insight into structural differences which underlie enzyme microheterogeneity.

机译：大肠杆菌β-半乳糖苷酶单个分子的电泳迁移率差异的测量提供了洞察酶微异质性基础的结构差异。

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The electrophoretic mobility and catalytic activity of individual molecules of Escherichia coli beta-galactosidase were measured using CE-LIF detection. Both the mobility and activity were reproducible for each molecule but differed between individual molecules. Assays were performed using uncoated capillaries and capillaries coated with different polymers, using enzymes from different sources and by three different experimental protocols. In all cases the observed ranges in electrophoretic mobilities were similar. The observed range in the electrophoretic mobility may be explained by structural microheterogeneity resulting in a gain or loss of up to 1.6 suppressed charge units. There was no observed relationship between the observed activities and electrophoretic mobilities. If the finding that individual beta-galactosidase molecules have heterogeneous electrophoretic mobility can be extended to other proteins, this may limit the resolution possible for capillary zone electrophoresis protein separations.

机译：大肠杆菌β-半乳糖苷酶单个分子的电泳迁移率和催化活性使用CE-LIF检测进行了测量。迁移率和活性对于每个分子都是可再现的，但是在单个分子之间是不同的。使用未涂覆的毛细管和涂覆有不同聚合物的毛细管，使用来自不同来源的酶并通过三种不同的实验方案进行测定。在所有情况下，观察到的电泳迁移率范围都相似。观察到的电泳迁移率范围可以用结构微异质性解释，该结构导致最多1.6个抑制电荷单元的得失。在观察到的活性和电泳迁移率之间没有观察到的关系。如果发现单个β-半乳糖苷酶分子具有不同的电泳迁移率的发现可以扩展到其他蛋白质，则这可能会限制毛细管区带电泳蛋白质分离的分辨率。

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《Electrophoresis: The Official Journal of the International Electrophoresis Society》 |2008年第20期|共13页
作者
Nichols ER; Craig DB;
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正文语种 eng
中图分类化学;
关键词
Escherichia coli; Measurement; Insight; NOS; Proteins; Enzymes; 大肠杆菌; 蛋白质类; 酶类;

机译：Escherichia coli;Measurement;Insight;NOS;Proteins;Enzymes;大肠杆菌;蛋白质类;酶类;

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1. Measurement of the differences in electrophoretic mobilities of individual molecules of E. coli beta-galactosidase provides insight into structural differences which underlie enzyme microheterogeneity. [J] . Nichols ER, Craig DB Electrophoresis: The Official Journal of the International Electrophoresis Society . 2008,第20期

机译：大肠杆菌β-半乳糖苷酶单个分子的电泳迁移率差异的测量提供了洞察酶微异质性基础的结构差异。
2. Measurement of the differences in electrophoretic mobilities of individual molecules of E. coli beta-galactosidase provides insight into structural differences which underlie enzyme microheterogeneity. [J] . Nichols ER, Craig DB Electrophoresis: The Official Journal of the International Electrophoresis Society . 2008,第20期

机译：大肠杆菌β-半乳糖苷酶单个分子的电泳迁移率差异的测量提供了洞察酶微异质性基础的结构差异。
3. Differences in the average single molecule activities of E. coli beta-galactosidase: effect of source, enzyme molecule age and temperature of induction. [J] . Craig DB, Nachtigall JT, Ash HL, Journal of Protein Chemistry . 2003,第6期

机译：大肠杆菌β-半乳糖苷酶的平均单分子活性的差异：来源，酶分子年龄和诱导温度的影响。
4. Effects of Strain Differences on Sorption of E. coli to Kaolinite [C] . Kate Lago, Daniel Gallagher, Charles Hagedorn III, Water quality technology conference and exposition (2005 WQTC conference proceedings) . 2005

机译：应变差异对大肠杆菌吸附高岭石的影响
5. Identification of genomic differences between Escherichia coli strains pathogenic for poultry and E. coli K-12 MG1655. [D] . Stocki, Stacy Lynn. 2003

机译：鉴定家禽致病性大肠杆菌菌株与大肠杆菌K-12 MG1655之间的基因组差异。
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7. Decision-making is the process of analyzing information about a problem situation and comparing it to a specific conclusion in order to solve a specific problematic (Yıkılmaz, 2001; Miller and Byrnes, 2001). Decision-making styles are a mechanism that is influenced by the internal and external conditions that determine the direction of the decisions of the individual, the content of the decision-making process, and the outcome of the decision-making process (Payne, Bettman and Johson, 1993; Bavol’ár and Orosová, 2015). ACT is a contemporary member of the Cognitive Behavioral Therapy family. ACT (Acceptance and commitment therapy) has both similar and different directions with Behavioral Therapies and Cognitive Therapies (Herbet and Forman, 2011; Hayes, 2004). KKT responds to classical behavioral treatments using both existential and cognitive approaches in the analysis of behavior. KKT is a science wing that tries to solve human problems with a wider perspective aimed at solving problematic human behaviors (Plumb, Stewart, Dahl and Lundgren, 2009). It is seen that there is very little research about the new approach of ACT approach when the aiming country of our country is screened and it is thought that our country will contribute to the field of psychological counseling with the work done. In the scope of the research, experimental and control groups and preliminary test, post-test and follow-up measurements of 2x3 experimental design were used. The study's study group consists of a total of 24 (12 experimental and 12 control groups) university students studying in different departments and levels, continuing their education in the academic year of 2015-2016 in Ağrı province and İbrahim Chechen University in 2015-2016 academic year. The average age of participants in the experiment and control group is 20. There were 12 participants in the experimental group, 7 female and 5 male, and 12 participants, 7 female and 5 male in the control group. Personal Information Form and Decision Making Style Scale prepared by the researcher were used in the research. In order to decide on the tests to be used in the course of analyzing the data, the scores of the participant's Decision Styles Scale pre-test, which are placed primarily in the experimental and control groups, it was analyzed whether the basic expectations of parametric tests were answered. As a result of the analysis made, the scores, skewness and kurtosis coefficients obtained from the Decision Making Styles Scale were given to the experimental and control groups. It was determined that the distribution was normal in the result of Shapiro-Wilk test, in which the skewness and kurtosis coefficients of each sub-scale were ranked between -1 and +1. Participants in the experimental and control groups; homogeneity test results for decision-style pre-test measurements indicate that the data are homogeneous. According to the results of the Mauchly Globalness Test, it has been determined that working supports the hypothesis. It was determined that there was no significant difference between the pre-test scores obtained from dependent decision-making style of experiment and control groups, but the test group showed lower mean scores at the significant level within the scores of post-test and follow-up tests. Therefore, it can be said that the ACT-oriented psychoeducation program applied to the experimental group reduces the dependent decision-making style scores from the decision style sub-dimensions and the psychoeducation program has a lasting effect. It was determined that there was no significant difference between pre-test, post-test and follow-up scores obtained from the Spontaneous-Instant Decision Style of experiment and control groups. Thus, it can be said that this situation does not cause a significant difference in the Spontaneous-Decision-Making Style scores from the decision style sub-dimensions of the ACT-oriented psychoeducation program applied to the experimental group. The ACT -oriented psychoeducation program had a decline in the intuitive decision-making styles of the individuals, but this decrease did not create significant differences. Thus, it can be said that this situation does not make a meaningful difference in the intuitive decision style scores from the decision style sub-dimensions of the KKT oriented psychoeducation program applied to the experimental group. The pre-test scores obtained from the rational decision-making style of the experimental and control groups showed that there was a difference between the post-test and the follow-up scores, but this difference was not significant. As a result of the analysis, it was determined that the test group had higher levels of rational decision style than the pre - test scores in the post test and follow - up scores, whereas the post test and follow - up test scores in the control group rational decision style showed a decrease compared to the pre - test scores. the pre - test scores. Decision-making Styles Scale Avoidant Decision Making As a result of the analysis of the mean scores of the subscale scores of pre-test, post-test and follow-up measures, the group effect was found to be insignificant. 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Measurement of the differences in electrophoretic mobilities of individual molecules of E. coli beta-galactosidase provides insight into structural differences which underlie enzyme microheterogeneity.

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