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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Investigation of C-reactive protein binding to phosphatidyl choline by CZE and ESI-mass analysis.
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Investigation of C-reactive protein binding to phosphatidyl choline by CZE and ESI-mass analysis.

机译：通过CZE和ESI-mass分析研究C反应蛋白与磷脂酰胆碱的结合。

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摘要

A simple CZE has been developed to investigate the binding reactions between C-reactive protein (CRP) and native as well as Cu(2+) oxidized phosphatidyl choline (PC) in this study. An aqueous-organic solvent system containing 20% sodium phosphate, 70% methanol and 10% acetonitrile was selected as the optimal CZE separation buffer. Native PC showed a broad, higher mobility major peak and a sharp, lower mobility minor peak. Oxidized PC (ox-PC) appeared as two adjacent peaks. PC bound to CRP in the presence of metal ions Ca(2+) and Mg(2+). The absorbance at 214 nm for PC+CRP was about five times of that for PC. Surprisingly, ox-PC interacted much more weakly with CRP. The absorbance at 214 nm for ox-PC+CRP was close to that of ox-PC. Meanwhile, ESI-Mass analysis also suggested that four PC molecular species (C16:0/C18:1, C16:0/C18:2, C18:0/C18:2, C18:0/C18:1) bound with CRP since the ion intensities of PC+CRP were lower than that of PC alone. This study uses both CZE and ESI-Mass analysis to characterize the interaction between CRP and native as well as Cu(2+) oxidized PC, and provides new analytical methods for determining the binding reactions. In addition, it also gives new insight into the molecular interactions between CRP and native as well as oxidized PC.
机译：已经开发了一种简单的CZE，用于研究C反应蛋白（CRP）与天然以及Cu（2+）氧化的磷脂酰胆碱（PC）之间的结合反应。选择包含20％磷酸钠，70％甲醇和10％乙腈的水性有机溶剂系统作为最佳的CZE分离缓冲液。本地PC表现出一个宽广的，较高迁移率的主峰和一个陡峭的，较低迁移率的次峰。氧化的PC（ox-PC）显示为两个相邻的峰。 PC绑定到CRP在金属离子Ca（2+）和Mg（2+）的存在下。 PC + CRP在214 nm处的吸光度约为PC的五倍。令人惊讶的是，ox-PC与CRP的相互作用弱得多。 ox-PC + CRP在214 nm处的吸光度接近ox-PC。同时，ESI-Mass分析还表明，四个PC分子种类（C16：0 / C18：1，C16：0 / C18：2，C18：0 / C18：2，C18：0 / C18：1）自PC + CRP的离子强度低于单独的PC。这项研究使用CZE和ESI-Mass分析来表征CRP与天然以及Cu（2+）氧化的PC之间的相互作用，并提供确定结合反应的新分析方法。此外，它也为CRP与天然以及氧化PC之间的分子相互作用提供了新的见解。

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