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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Insulator-based dielectrophoresis of microorganisms: theoretical and experimental results.
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Insulator-based dielectrophoresis of microorganisms: theoretical and experimental results.

机译：基于绝缘子的微生物介电泳：理论和实验结果。

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摘要

Dielectrophoresis (DEP) is the motion of particles due to polarization effects in nonuniform electric fields. DEP has great potential for handling cells and is a non-destructive phenomenon. It has been utilized for different cell analysis, from viability assessments to concentration enrichment and separation. Insulator-based DEP (iDEP) provides an attractive alternative to conventional electrode-based systems; in iDEP, insulating structures are used to generate nonuniform electric fields, resulting in simpler and more robust devices. Despite the rapid development of iDEP microdevices for applications with cells, the fundamentals behind the dielectrophoretic behavior of cells has not been fully elucidated. Understanding the theory behind iDEP is necessary to continue the progress in this field. This work presents the manipulation and separation of bacterial and yeast cells with iDEP. A computational model in COMSOL Multiphysics was employed to predict the effect of direct current-iDEP on cells suspended in a microchannel containing an array of insulating structures. The model allowed predicting particle behavior, pathlines and the regions where dielectrophoretic immobilization should occur. Experimental work was performed at the same operating conditions employed with the model and results were compared, obtaining good agreement. This is the first report on the mathematical modeling of the dielectrophoretic response of yeast and bacterial cells in a DC-iDEP microdevice.
机译：介电电泳（DEP）是由于非均匀电场中的极化效应而引起的粒子运动。 DEP具有处理细胞的巨大潜力，是一种非破坏性现象。它已用于不同的细胞分析，从生存能力评估到浓度富集和分离。基于绝缘体的DEP（iDEP）提供了一种有吸引力的替代方案，可替代传统的基于电极的系统。在iDEP中，绝缘结构用于产生不均匀的电场，从而使设备更简单，更坚固。尽管用于细胞应用的iDEP微型设备发展迅速，但细胞介电行为背后的基本原理尚未得到充分阐明。理解iDEP背后的理论对于继续该领域的进步是必不可少的。这项工作介绍了iDEP的细菌和酵母细胞的操作和分离。使用COMSOL Multiphysics中的计算模型来预测直流iDEP对悬浮在包含绝缘结构阵列的微通道中的细胞的影响。该模型可以预测粒子的行为，路径和介电泳固定的区域。在与模型相同的操作条件下进行了实验工作，并对结果进行了比较，取得了很好的一致性。这是关于DC-iDEP微型设备中酵母和细菌细胞介电电泳反应数学建模的第一份报告。

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