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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Direct current insulator-based dielectrophoretic characterization of erythrocytes: ABO-Rh human blood typing.
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Direct current insulator-based dielectrophoretic characterization of erythrocytes: ABO-Rh human blood typing.

机译：基于直流电绝缘子的红细胞介电泳特性：ABO-Rh人血型。

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摘要

A microfluidic platform developed for quantifying the dependence of erythrocyte (red blood cell, RBC) responses by ABO-Rh blood type via direct current insulator dielectrophoresis (DC-iDEP) is presented. The PDMS DC-iDEP device utilized a 400 x 170 mum(2) rectangular insulating obstacle embedded in a 1.46-cm long, 200-mum wide inlet channel to create spatial non-uniformities in direct current (DC) electric field density realized by separation into four outlet channels. The DC-iDEP flow behaviors were investigated for all eight blood types (A+, A-, B+, B-, AB+, AB-, O+, O-) in the human ABO-Rh blood typing system. Three independent donors of each blood type, same donor reproducibility, different conductivity buffers (0.52-9.1 mS/cm), and DC electric fields (17.1-68.5 V/cm) were tested to investigate separation dependencies. The data analysis was conducted from image intensity profiles across inlet and outlet channels in the device. Individual channel fractions suggest that the dielectrophoretic force experienced by the cells is dependent on erythrocyte antigen expression. Two different statistical analysis methods were conducted to determine how distinguishable a single blood type was from the others. Results indicate that channel fraction distributions differ by ABO-Rh blood types suggesting that antigens present on the erythrocyte membrane polarize differently in DC-iDEP fields. Under optimized conductivity and field conditions, certain blind blood samples could be sorted with low misclassification rates.
机译：提出了一种微流体平台，该平台用于通过直流绝缘子介电电泳（DC-iDEP）量化ABO-Rh血型对红细胞（红细胞，RBC）反应的依赖性。 PDMS DC-iDEP设备利用嵌入在1.46厘米长，200毫米宽的入口通道中的400 x 170 mum（2）矩形绝缘障碍物，通过分离实现直流（DC）电场密度的空间不均匀性分为四个出口渠道。研究了人类ABO-Rh血液分型系统中所有八种血液类型（A +，A-，B +，B-，AB +，AB-，O +，O-）的DC-iDEP流动行为。测试了每种血液类型的三个独立供体，相同的供体再现性，不同的电导率缓冲液（0.52-9.1 mS / cm）和直流电场（17.1-68.5 V / cm），以研究分离依赖性。数据分析是通过跨设备入口和出口通道的图像强度分布进行的。各个通道的分数表明细胞所经历的介电泳力取决于红细胞抗原的表达。进行了两种不同的统计分析方法，以确定一种血型与其他血型的可区分性。结果表明通道分数分布因ABO-Rh血型而异，这表明存在于DC-iDEP字段中的红细胞膜上的抗原极化方式不同。在优化的电导率和田间条件下，可以以低误分类率对某些盲血样本进行分类。

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