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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >ANALYSIS OF PROTEINS FROM HUMAN BREAST EPITHELIAL CELLS USING TWO-DIMENSIONAL GEL ELECTROPHORESIS
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ANALYSIS OF PROTEINS FROM HUMAN BREAST EPITHELIAL CELLS USING TWO-DIMENSIONAL GEL ELECTROPHORESIS

机译：二维凝胶电泳分析人乳腺上皮细胞中的蛋白质

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The human breast is a highly specialized, complex tissue comprised of a heterogeneous population of cells with varying functions. Interactions between the different cell types, changes in their relative abundance, state of differentiation and function in response to stimuli, as well as the alterations that lead to the aberrant growth associated with malignancy are poorly understood. Two-dimensional gel electrophoresis is being used to compare the proteins found in different breast cells in order to identify the gene products that are common or specific to particular cell types so as to provide markers that will be useful in studies of normal breast cell differentiation and the dedifferentiation or blocked differentiation characteristic of cancer. Protein patterns have been obtained from cells prepared for electrophoresis immediately after isolation from human milk, from cells cultured for fewer than ten passages after isolation from healthy breast tissue removed during reduction mammoplasty, and from cells maintained in long-term tissue culture after isolation from the pleural effusions of patients with breast carcinomas. Differential expression of cytokeratins 8, 18, and 19, shown previously to be predominantly expressed by epithelial cells in the luminal layer of breast tissue, was observed among the cells analyzed. Other non-cytokeratin proteins were also found to be differentially expressed in subsets of both the normal and tumor cells. A composite human breast cell protein pattern was created which includes all the commonly and specifically expressed proteins found in this study. This pattern will be the basis for continuing studies of proteins in the human breast. [References: 31]
机译：人的乳房是高度专业化的复杂组织，由功能各异的细胞异质群体组成。人们很少了解不同细胞类型之间的相互作用，其相对丰度的变化，分化状态和功能对刺激的反应以及导致与恶性肿瘤相关的异常生长的改变。二维凝胶电泳用于比较在不同乳腺细胞中发现的蛋白质，以鉴定特定细胞类型共有或特异的基因产物，从而提供可用于正常乳腺细胞分化和分化研究的标记物。癌症的去分化或阻断分化特征。从人乳中分离后立即为电泳准备的细胞，在还原乳头成形术中取出的健康乳房组织中分离后培养少于十代的细胞以及从中分离后在长期组织培养中维持的细胞均已获得蛋白质图谱。乳腺癌患者的胸腔积液。在所分析的细胞中，观察到细胞角蛋白8、18和19的差异表达，先前已证明主要由乳腺组织腔层中的上皮细胞表达。还发现其他非细胞角蛋白蛋白质在正常细胞和肿瘤细胞的亚组中差异表达。创建了一种复合人乳腺细胞蛋白质模式，其中包括本研究中发现的所有常见和特异表达的蛋白质。这种模式将成为继续研究人类乳房蛋白质的基础。 [参考：31]

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