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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Determination of phytic acid and inositolphosphates in barley.
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Determination of phytic acid and inositolphosphates in barley.

机译:大麦中植酸和肌醇磷酸的测定。

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摘要

Phytic acid (PA) and lower inositolphosphates (InsP(n) ) is the main storage form of phosphorus in grains or seeds. The content of PA and InsP(n) in different varieties of barley was analyzed by capillary isotachophoresis and online-coupled capillary isotachophoresis with CZE. The electrolytes (in demineralized water) for the isotachophoretic analysis consisted of 10 mM HCl, 14 mM glycylglycine, and 0.1% 2-hydroxyethylcellulose (leading) and 10 mM citric acid (terminating). The optimized electrolytes for the online coupling isotachophoresis with zone electrophoresis analysis were mixtures of 5 mM HCl, 7 mM glycylglycine, and 0.1% 2-hydroxyethylcellulose (leading), 20 mM citric acid, 10 mM glycylglycine, and 0.1% 2-hydroxyethylcellulose (background) and 10 mM citric acid (terminating). PA and all studied InsP(n) were separated within 25 min and detected by a conductivity detector. Simple sample preparation (acidic extraction), sufficient sensitivity, speed of analysis, and low running cost are important attributes of the electrophoretic methods. The method was used for the determination of PA and InsP(n) in barley varieties within an ongoing research project.
机译:植酸(PA)和低级肌醇磷酸酯(InsP(n))是谷物或种子中磷的主要存储形式。通过毛细管等速电泳和CZE在线耦合毛细管等速电泳分析了大麦不同品种中PA和InsP(n)的含量。用于等位电泳分析的电解质(在软化水中)由10 mM HCl,14 mM甘氨酰甘氨酸和0.1%2-羟乙基纤维素(前导)和10 mM柠檬酸(终止)组成。用于在线耦合等速电泳和区域电泳分析的最佳电解质是5 mM HCl,7 mM甘氨酰甘氨酸和0.1%2-羟乙基纤维素(前导),20 mM柠檬酸,10 mM甘氨酰甘氨酸和0.1%2-羟乙基纤维素(本底)的混合物)和10 mM柠檬酸(终止)。 PA和所有研究的InsP(n)在25分钟内分离,并由电导检测器检测。简单的样品制备(酸性提取),足够的灵敏度,分析速度和低运行成本是电泳方法的重要属性。在正在进行的研究项目中,该方法用于测定大麦品种中的PA和InsP(n)。

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