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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >A method to determine quasi-steady state in constant voltage mode isotachophoresis.
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A method to determine quasi-steady state in constant voltage mode isotachophoresis.

机译：一种在恒压模式等速电泳中确定准稳态的方法。

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摘要

Identification of the steady state is very challenging in isotachophoresis (ITP); especially in complex microgeometries, such as dog-leg channels or cross-channel junctions. In this work, an elastic matching method is applied to determine the quasi-steady state in microscale ITP. In the elastic matching method, the similarity between two profiles is calculated by comparing intensity distribution of two images or profiles. To demonstrate this similarity-based analysis technique for ITP, a constant voltage mode ITP model is developed and applied to a five-component ITP system. Hydrochloric acid and caproic acid are used as the leader and terminator, respectively, while histidine is used as the counter-ion. Two sample components, acetic acid and benzoic acid, are separated under the action of an applied electric field in both straight and dog-leg microchannels. This analysis shows that conductivity profiles provide a better measure to determine the quasi-steady state in an ITP process. For a straight microchannel, the quasi-steady state is achieved in less than a minute with a total potential drop of 100 V in a 2 cm long channel. In a straight channel, a true steady state can be achieved for ITP with appropriate countercurrent flow where stationary zones are formed, but the time it takes to reach the steady state is much longer than the without counter flow case. The numerical results indicate that a steady state cannot be reached in a dog-leg microchannel because of sample dispersion and refocusing at and near the intersections and at the branch channels. However, the elastic matching method can be used to determine the quasi-steady state in a dog-leg microchannel.
机译：在等速电泳（ITP）中，鉴定稳态是非常具有挑战性的。特别是在复杂的微几何形状中，例如狗腿通道或跨通道连接处。在这项工作中，采用弹性匹配方法来确定微尺度ITP中的准稳态。在弹性匹配方法中，通过比较两个图像或轮廓的强度分布来计算两个轮廓之间的相似度。为了演示这种基于相似度的ITP分析技术，开发了恒定电压模式ITP模型并将其应用于五组件ITP系统。盐酸和己酸分别用作前导剂和终止剂，而组氨酸用作抗衡离子。在直电场和狗腿微通道中，在施加电场的作用下，乙酸和苯甲酸这两种样品组分被分离。该分析表明，电导率分布图为确定ITP过程中的准稳态提供了更好的方法。对于直的微通道，在不到一分钟的时间内即可达到准稳态，而在2 cm长的通道中总电位降为100V。在直通道中，具有适当的逆流流动的ITP可以在形成固定区域的情况下达到ITP的真正稳态，但是达到稳态所需的时间比没有逆流情况下要长得多。数值结果表明，由于样本分散以及在交叉点处和分支点附近以及分支通道处重新聚焦，无法在狗腿微通道中达到稳态。但是，弹性匹配方法可用于确定狗腿微通道中的准稳态。

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