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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Insulator-based dielectrophoretic single particle and single cancer cell trapping.
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Insulator-based dielectrophoretic single particle and single cancer cell trapping.

机译：基于绝缘体的介电泳单粒子和单癌细胞捕获。

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摘要

Trapping of individual cells at specific locations in a microfluidic lab-on-a-chip platform is essential for single cell studies, especially those requiring individual stimulation followed by downstream analysis. To this aim, we have designed microdevices based on direct current (DC) insulator-based dielectrophoresis (iDEP) acting as individual single cell traps. We present both the design of a negative iDEP trap and a positive iDEP trap using insulating posts integrated at microchannel intersections. We obtained electric field distributions via numerical simulations adapted to the intersection and trap geometry with which we predict single particle pathlines. With polystyrene particles of 10 mum diameter, we demonstrated an effective design for a single particle trap in the case of negative dielectrophoresis. The onset trapping voltage shows an inverse relation to the buffer conductivity, thus indicating the influence of electrokinetic effects on the trapping behavior. Additionally, we demonstrated the proof-of-principle of single MCF-7 breast cancer cell trapping in a positive iDEP trap. Our single particle trapping experiments were further in very good agreement with numerical simulations. To ensure that no significant damage occurred to the cells during the experiment, we further optimized medium conditions to ensure viability of the cells for at least 1 h, more than sufficient for microfluidic trapping experiments. Our results thus indicated the successful design of DC iDEP traps, which can easily be integrated into a variety of microchip operations for single cell analysis.
机译：在微流控芯片实验室平台中的特定位置捕获单个细胞对于单细胞研究至关重要，尤其是那些需要单个刺激后进行下游分析的研究。为此，我们设计了基于直流（DC）绝缘子基介电电泳（iDEP）的微器件，这些器件充当单个单细胞陷阱。我们介绍了使用集成在微通道交叉处的绝缘柱设计的负iDEP阱和正iDEP阱的设计。我们通过数值模拟获得了电场分布，该数值模拟适用于我们预测单个粒子路径的相交和陷阱几何形状。对于直径为10毫米的聚苯乙烯颗粒，我们证明了在负介电电泳情况下单个颗粒阱的有效设计。起始捕获电压与缓冲液电导率呈反比关系，因此表明了电动效应对捕获行为的影响。此外，我们证明了在阳性iDEP阱中捕获单个MCF-7乳腺癌细胞的原理证明。我们的单粒子捕获实验与数值模拟进一步吻合得很好。为了确保在实验过程中不会对细胞造成明显损害，我们进一步优化了培养基条件，以确保细胞存活至少1小时，这对于微流体捕集实验来说绰绰有余。因此，我们的结果表明成功设计了DC iDEP阱，该阱可轻松集成到各种微芯片操作中以进行单细胞分析。

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