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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Scattering detection using a photonic-microfluidic integrated device with on-chip collection capabilities
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Scattering detection using a photonic-microfluidic integrated device with on-chip collection capabilities

机译：使用具有芯片上采集功能的光子-微流体集成设备进行散射检测

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摘要

SU-8-based photonic-microfluidic integrated devices with on-chip beam shaping and collection capabilities were demonstrated in a scattering detection and counting application. Through the proper deployment of the tailored beam geometries via the on-chip excitation optics, excellent CV values were measured for 1, 2, and 5 μm blank beads, 16.4, 11.0, and 12.5%, respectively, coupled with a simple free-space optical detection scheme. The performance of these devices was found dependent on the combination of on-chip, lens-shaped beam geometry and bead size. While very low CVs were obtained when the combination was ideal, a nonideal combination could still result in acceptable CVs for flow cytometry; the reliability was confirmed via devices being able to resolve separate populations of 2.0 and 5.0 μm beads from their mixture with low CV values of 15.9 and 18.5%, respectively. On-chip collection using integrated on-chip optical waveguides was shown to be very reliable in comparison with a free-space collection scheme, yielding a coincident rate of 94.2%. A CV as low as 19.2% was obtained from the on-chip excitation and collection of 5 μm beads when the on-chip lens-shaped beam had a 6.0-μm beam waist.
机译：具有SU的光子微流控集成设备具有片上光束成形和收集功能，已在散射检测和计数应用中得到了证明。通过片上激发光学器件适当地调整定制的光束几何形状，分别测量了1、2和5μm的空白磁珠（分别为16.4、11.0和12.5％）的出色CV值，以及简单的自由空间光学检测方案。发现这些器件的性能取决于片上透镜形状的光束几何形状和磁珠尺寸的组合。当理想的组合获得了非常低的CV时，非理想的组合仍然可以为流式细胞术带来可接受的CV。通过能够从其混合物中分别分离出2.0和5.0μm珠子的CV值分别为15.9和18.5％的低值，证实了可靠性。与自由空间收集方案相比，使用集成的片上光波导进行的片上收集被证明是非常可靠的，重合率为94.2％。当片上透镜形光束的束腰为6.0μm时，通过片上激发和收集5μm磁珠可得到低至19.2％的CV。

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