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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >On-capillary derivatisation as an approach to enhancing sensitivity in capillary electrophoresis
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On-capillary derivatisation as an approach to enhancing sensitivity in capillary electrophoresis

机译：毛细管上衍生化作为增强毛细管电泳灵敏度的方法

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摘要

Separation technologies play an important role in revealing biological processes at various omic levels, in pharmacological and clinical research. In this context, CE is a strong candidate for analyses of samples with rapidly increasing complexity. Even though CE is well known for its many advantages in this regard, the sensitivity of CE analyses is insufficient for many applications. Accordingly, there are generally three main options for enhancing the sensitivity of CE analyses - using special detection techniques, using sample pre-concentration and derivatisation. Derivatisation is often the method of choice for many laboratories, since it is simple and provides several advantages such as small sample volume demand and the possibility of automation. Although it can be performed in different ways depending on where the reaction takes place, this article reviews one of the simplest and at the same time most useful approaches on-capillary derivatisation. Even if in many cases the use of on-capillary derivatisation alone is enough to improve the detection sensitivity, on other occasions it needs to be employed in combination with the other above-mentioned strategies. After a simple discussion of derivatisation in general, special attention is focused on the on-capillary approach and methodologies available for on-capillary reactant mixing. Its applications in various fields are also described.
机译：在药理学和临床研究中，分离技术在揭示不同组学水平的生物过程中起着重要作用。在这种情况下，CE是分析复杂性迅速增加的样品的理想选择。尽管CE在这方面具有许多优势而闻名，但CE分析的灵敏度不足以用于许多应用。因此，通常有三个主要选项可用于增强CE分析的灵敏度-使用特殊的检测技术，使用样品预浓缩和衍生化。衍生化通常是许多实验室的首选方法，因为它简单易行，并提供了许多优点，例如样品量需求少和自动化的可能性。尽管可以根据发生反应的方式以不同的方式进行，但本文还是对毛细管衍生的最简单，同时也是最有用的方法之一进行了综述。即使在许多情况下，仅使用毛细管上的衍生作用足以改善检测灵敏度，在其他情况下，也需要将其与其他上述策略结合使用。在一般性地简单讨论衍生化之后，应特别注意毛细管内方法和可用于毛细管内反应物混合的方法。还介绍了它在各个领域的应用。

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