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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >A glycopeptide antibiotic chiral stationary phase for the enantiorner resolution of hydroxy acid derivatives by capillary electrochromatography
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A glycopeptide antibiotic chiral stationary phase for the enantiorner resolution of hydroxy acid derivatives by capillary electrochromatography

机译：一种糖肽抗生素手性固定相，用于通过毛细管电色谱法拆分羟酸衍生物的对映体

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摘要

Separation of hydroxy acid enantiomers was achieved by using capillary electrochromatography (CEC) employing a chiral stationary phase (CSP) based on MDL 63,246 (Hepta-Tyr), a macrocyclic antibiotic of the teicoplanin family. The chiral selector was chemically bonded to 5 mum diol-modified silica particles and the CSP mixed with amino silica (3:1 w/w) was packed into a 75 mum ID fused-silica capillary. The CEC experiments were carried out by using an aqueous reversed-phase mode for the enantiomeric resolution of hydroxy acid compounds. Good enantioresolution was achieved for mandelic acid (MA), m-hydroxymandelic acid (m-OH-MA), p-OH-MA, and 3-hydroxy-4-methoxymandelic acid (3-OH-4-MeO-MA). The CEC system was less enantioselective towards 2-phenyllactic acid (2-PhL) and 3-PhL while mandelic acid methyl ester (MA-Et-Est) enantiomers were not resolved. Several experimental parameters, such as organic solvent type and concentration, buffer pH, capillary temperature, on enantioresolution factor, retention time, and retention factor were studied. [References: 36]
机译：羟基酸对映异构体的分离是通过使用毛细管电色谱（CEC）进行的，该毛细管电色谱基于手性固定相（CSP），该手性基于替丁普林家族的大环抗生素MDL 63,246（Hepta-Tyr）。将手性选择剂化学键合到5um二醇改性的二氧化硅颗粒上，然后将与氨基二氧化硅（3：1 w / w）混合的CSP填充到75um ID熔融石英毛细管中。通过使用水性反相模式进行CEC实验，以分离羟基酸化合物的对映体。对扁桃酸（MA），间羟基扁桃酸（m-OH-MA），对羟基酚-MA和3-羟基-4-甲氧基扁桃酸（3-OH-4-MeO-MA）实现了良好的对映拆分。 CEC系统对2-苯基乳酸（2-PhL）和3-PhL的对映选择性较低，而扁桃酸甲酯（MA-Et-Est）对映体尚未分离。研究了几种实验参数，例如有机溶剂的类型和浓度，缓冲液pH，毛细管温度，对映体分离因子，保留时间和保留因子。 [参考：36]

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