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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >A comparison between electrokinetic capillary chromatography and absorption spectroscopy for the analysis of peptide-micelle association by weak hydrophobic interactions
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A comparison between electrokinetic capillary chromatography and absorption spectroscopy for the analysis of peptide-micelle association by weak hydrophobic interactions

机译：电动毛细管色谱法和吸收光谱法通过弱疏水相互作用分析肽-胶束缔合的比较

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摘要

Micellar electrokinetic capillary chromatography (MEKC) was compared to absorption spectroscopy to estimate equilibrium association constans (K-as) for peptide-micelle systems involving three peptides (leucine-enkephalin, methionine-enkephalin and leucine-phenylalanine (LF)) and two surfactant micelles; (sodium dodecyl sulfate (SDS) and cetyltrimethylammonium bromide (CTAB)). Buffer pH was chosen to minimize purely electrostatic interactions between peptides and micelles that could not be interrogated by absorption spectroscopy. Viscosity-corrected MEKC mobilities gave reasonably similar estimates of K-as between the two methods for all three peptide-SDS micelle systems, with K-as values ranging from 13.7 +/- 0.3 to 49 +/- 1 M-1. For CTAB, estimates of K-as for LF-CTAB micelle association were of the same order of magnitude as the SDS micelle by the two methods of estimation. On the other hand, enkephalin-CTAB micelle binding was about 10 times stronger (K-as = 122 +/- 3 M-1 to 311 +/- 9 M-1) than the enkephalin-SDS micelle binding. In addition, MEKC underestimated the K-as values relative to spectroscopy by a factor of 2-3 for the enkephalin-CTAB system. [References: 31]
机译：将胶束电动毛细管色谱法（MEKC）与吸收光谱法进行比较，以评估涉及三个肽（亮氨酸-脑啡肽，蛋氨酸-脑啡肽和亮氨酸-苯丙氨酸（LF））和两个表面活性剂胶束的肽-胶束系统的平衡缔合常数（K-as）。 ; （十二烷基硫酸钠（SDS）和十六烷基三甲基溴化铵（CTAB））。选择缓冲液pH值以最大程度地减少肽和胶束之间纯净的静电​​相互作用，而这无法通过吸收光谱法进行询问。对于所有三种肽-SDS胶束系统，两种方法之间的粘度校正后的MEKC迁移率都给出了合理的K-as估计值，K-as值范围从13.7 +/- 0.3到49 +/- 1 M-1。对于CTAB，通过两种估计方法，对于LF-CTAB胶束缔合的K-as估计与SDS胶束的数量级相同。另一方面，脑啡肽-CTAB胶束结合比脑啡肽-SDS胶束结合强约10倍（K-as = 122 +/- 3 M-1至311 +/- 9 M-1）。另外，对于脑啡肽-CTAB系统，MEKC相对于光谱法低估了K-as值2-3倍。 [参考：31]

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