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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Microfluidic chip-based liquid chromatography coupled to mass spectrometry for determination of small molecules in bioanalytical applications
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Microfluidic chip-based liquid chromatography coupled to mass spectrometry for determination of small molecules in bioanalytical applications

机译：基于微流控芯片的液相色谱仪与质谱联用，用于生物分析应用中的小分子测定

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摘要

The development and integration of microfabricated liquid chromatography (LC) microchips have increased dramatically in the last decade due to the needs of enhanced sensitivity and rapid analysis as well as the rising concern on reducing environmental impacts of chemicals used in various types of chemical and biochemical analyses. Recent development of microfluidic chip-based LC mass spectrometry (chip-based LC-MS) has played an important role in proteomic research for high throughput analysis. To date, the use of chip-based LC-MS for determination of small molecules, such as biomarkers, active pharmaceutical ingredients (APIs), and drugs of abuse and their metabolites, in clinical and pharmaceutical applications has not been thoroughly investigated. This mini-review summarizes the utilization of commercial chip-based LC-MS systems for determination of small molecules in bioanalytical applications, including drug metabolites and disease/tumor-associated biomarkers in clinical samples as well as adsorption, distribution, metabolism, and excretion studies of APIs in drug discovery and development. The different types of commercial chip-based interfaces for LC-MS analysis are discussed first and followed by applications of chip-based LC-MS on biological samples as well as the comparison with other LC-MS techniques.
机译：在过去的十年中，由于对增强灵敏度和快速分析的需求以及对减少各种化学和生化分析中所用化学物质对环境影响的关注日益增加，微细液相色谱（LC）微芯片的开发和集成已大大增加。 。基于微流控芯片的液相色谱质谱仪（基于芯片的LC-MS）的最新发展在蛋白质组学研究中为高通量分析发挥了重要作用。迄今为止，在临床和制药应用中，基于芯片的LC-MS用于确定小分子（例如生物标志物，活性药物成分（API），滥用药物及其代谢产物）的用途尚未得到彻底研究。这份小型综述总结了基于商业芯片的LC-MS系统在生物分析应用中测定小分子的应用，包括临床样品中的药物代谢物和疾病/肿瘤相关生物标志物以及吸附，分布，代谢和排泄研究药物研发中的原料药首先讨论了用于LC-MS分析的不同类型的商用基于芯片的接口，然后讨论了基于芯片的LC-MS在生物样品上的应用以及与其他LC-MS技术的比较。

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