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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Assessment of cell viability after manipulation with insulator-based dielectrophoresis
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Assessment of cell viability after manipulation with insulator-based dielectrophoresis

机译：基于绝缘子的介电泳操作后的细胞活力评估

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摘要

The effects of insulator-based DEP (iDEP) manipulation on cell viability were investigated by varying operating conditions and the shape of the insulating structures. Experiments were conducted with Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae cells by varying the applied potential (300-1000 V), exposure time (1-4 min), and composition of the suspending medium (0-10% glucose); using devices made from polydimethylsiloxane. Cell viability was quantified employing Trypan blue staining protocols. The results illustrated a strong decrease in cell survival at higher applied electric potentials and exposure times; and an increase in cell viability obtained by increasing suspending medium osmolality. The composition and structure of the cell wall also played a major role on cell survival, where prokaryotic Gram-positive B. subtilis was the most resilient cell strain, while eukaryotic S. cerevisiae had the lowest survival rate. Due to the popularity of iDEP in applications with biological cells, characterizing how iDEP operating conditions affect cell viability is essential.
机译：通过改变操作条件和绝缘结构的形状，研究了基于绝缘子的DEP（iDEP）操作对细胞活力的影响。通过改变施加电势（300-1000 V），暴露时间（1-4分钟）和悬浮培养基的组成（0-10％葡萄糖），对大肠杆菌，枯草芽孢杆菌和酿酒酵母进行实验。使用由聚二甲基硅氧烷制成的设备。使用锥虫蓝染色方案定量细胞活力。结果表明，在较高的施加电势和暴露时间下，细胞存活率大大降低;并且通过增加悬浮培养基的重量克分子渗透压浓度而获得的细胞活力的增加。细胞壁的组成和结构在细胞存活中也起着重要作用，其中原核革兰阳性枯草芽孢杆菌是最有弹性的细胞株，而真核酿酒酵母的存活率最低。由于iDEP在生物细胞应用中的普及，因此表征iDEP操作条件如何影响细胞活力至关重要。

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