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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Adaption of a fragment analysis technique to an automated high-throughput multicapillary electrophoresis device for the precise qualitative and quantitative characterization of microbial communities
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Adaption of a fragment analysis technique to an automated high-throughput multicapillary electrophoresis device for the precise qualitative and quantitative characterization of microbial communities

机译：片段分析技术适用于自动化的高通量多毛细管电泳设备，可对微生物群落进行精确的定性和定量表征

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The analysis of microbial communities is of increasing importance in life sciences and bioengineering. Traditional techniques of investigations like culture or cloning methods suffer from many disadvantages. They are unable to give a complete qualitative and quantitative view of the total amount of microorganisms themselves, their interactions among each other and with their environment. Obviously, the determination of static or dynamic balances among microorganisms is of fast growing interest. The generation of species specific and fluorescently labeled 16S ribosomal DNA (rDNA) fragments by the terminal restriction fragment length polymorphism (T-RFLP) technique is a suitable tool to overcome the problems other methods have. For the separation of these fragments polyacrylamide gel sequencers are preferred as compared to capillary sequencers using linear polymers until now because of their higher electrophoretic resolution and therefore sizing accuracy. But modern capillary sequencers, especially multicapillary sequencers, offer an advanced grade of automation and an increased throughput necessary for the investigation of complex communities in long-time studies. Therefore, we adapted a T-RFLP technique to an automated high-throughput multicapillary electrophoresis device (ABI 3100 Genetic Analysis(R)) with regard to a precise qualitative and quantitative characterization of microbial communities. [References: 17]
机译：在生命科学和生物工程中，微生物群落的分析越来越重要。传统的调查技术（例如培养或克隆方法）有许多缺点。他们无法对微生物本身的总量，彼此之间的相互作用以及与环境的相互作用给出完整的定性和定量分析。显然，确定微生物之间静态或动态平衡的兴趣迅速增长。通过末端限制性片段长度多态性（T-RFLP）技术生成物种特异性的并荧光标记的16S核糖体DNA（rDNA）片段，是克服其他方法所存在问题的合适工具。迄今为止，与使用线性聚合物的毛细管测序仪相比，对于这些片段的分离，聚丙烯酰胺凝胶测序仪是优选的，因为它们具有更高的电泳分辨率，因此具有上浆精度。但是现代的毛细管测序仪，尤其是多毛细管测序仪，提供了高级自动化水平，并提高了长期研究中对复杂群落的研究所必需的通量。因此，就微生物群落的精确定性和定量表征而言，我们将T-RFLP技术应用于自动化的高通量多毛细管电泳设备（ABI 3100 Genetic Analysis（R））。 [参考：17]

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