...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Differential dielectric responses of chondrocyte and Jurkat cells in electromanipulation buffers
【24h】

Differential dielectric responses of chondrocyte and Jurkat cells in electromanipulation buffers

机译：电操纵缓冲液中软骨细胞和Jurkat细胞的差异介电响应

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Electromanipulation of cells as a label-free cell manipulation and characterization tool has gained particular interest recently. However, the applicability of electromanipulation, particularly dielectrophoresis (DEP), to biological cells is limited to cells suspended in buffers containing lower amounts of salts relative to the physiological buffers. One might question the use of low conductivity buffers (LCBs) forDEP separation, as cells are stressed in buffers lacking physiological levels of salt. In LCB, cells leak ions and undergo volume regulation. Therefore, cells exhibit time-dependent DEP response in LCB. In this work, cellular changes in LCB are assessed by dielectric spectroscopy, cell viability assay, and gene expression of chondrocytes and Jurkats. Results indicate leakage of ions from cells, increases in cytoplasmic conductivity, membrane capacitance, and conductance. Separability factor, which defines optimum conditions for DEP cell separation, for the two cell types is calculated using the cellular dielectric data. Optimum DEP separation conditions change as cellular dielectric properties evolve in LCB. Genetic analyses indicate no changes in expression of ionic channel proteins for chondrocytes suspended in LCB. Retaining cellular viability might be important during dielectrophoretic separation, especially when cells are to be biologically tested at a downstream microfluidic component.
机译：作为无标记细胞操纵和表征工具的细胞电操纵最近引起了特别的兴趣。然而，电操纵，特别是介电电泳（DEP）对生物细胞的适用性限于悬浮在缓冲液中的细胞，所述缓冲液相对于生理缓冲液含有较少量的盐。人们可能会质疑使用低电导率缓冲液（LCB）进行DEP分离，因为细胞在缺乏生理盐浓度的缓冲液中压力很大。在LCB中，细胞会泄漏离子并进行体积调节。因此，细胞在LCB中表现出时间依赖性的DEP响应。在这项工作中，LCB的细胞变化通过介电谱，细胞生存力测定以及软骨细胞和Jurkats的基因表达来评估。结果表明离子从细胞泄漏，细胞质电导率，膜电容和电导率增加。使用细胞介电数据计算两种细胞类型的DEF细胞分离条件，该因子定义了DEP细胞分离的最佳条件。最佳的DEP分离条件随着LCB中细胞介电性能的变化而变化。遗传分析表明，悬浮在LCB中的软骨细胞的离子通道蛋白表达没有变化。在介电泳分离过程中，保持细胞活力可能很重要，特别是当要在下游微流体组件进行生物学测试的细胞时。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号