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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Analysis of the performance of antibody capture methods using fluorescent peptides with capillary zone electrophoresis with laser-induced fluorescence
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Analysis of the performance of antibody capture methods using fluorescent peptides with capillary zone electrophoresis with laser-induced fluorescence

机译：荧光肽的毛细管电泳带激光诱导荧光的抗体捕获方法的性能分析

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摘要

A method to analyze the performance of an antibody capture method using fluorescent peptides by capillary zone electrophoresis using laser-induced fluorescence (CZE-LIF) for detection has been developed. Fluorescent peptides from the prion protein were synthesized and the corresponding antibodies were produced in rabbits against these peptides. The antibodies were used to capture the fluorescent peptides. The antibodies were then bound to protein A Sepharose. After elution, the amount of fluorescent peptide that was captured vs. the total amount placed in the assay was evaluated by CZE-LIF. Of the three peptides used in this evaluation, it was found that the recovery was approximately 25-35%. When the abnormal prion protein was prepared from scrapie-infected brain samples from hamsters and a sheep using the previously described extraction method and this method, the amount of abnormal prion protein that was measured in the fluorescence immunoassay correlated with amounts estimated from Western blot. We conclude that this method can be used to detect abnormal prion protein in a tissue sample. [References: 20]
机译：已经开发了一种通过使用激光诱导荧光（CZE-LIF）进行毛细管区带电泳来检测使用荧光肽的抗体捕获方法的性能的方法。合成了来自ion病毒蛋白的荧光肽，并在兔中产生了针对这些肽的相应抗体。抗体用于捕获荧光肽。然后将抗体结合至蛋白A琼脂糖。洗脱后，通过CZE-LIF评估捕获的荧光肽的量与放置在测定中的总量的比。在该评估中使用的三种肽中，发现回收率约为25-35％。当使用前述提取方法和该方法从仓鼠和绵羊的瘙痒病感染的脑样本中制备异常pr病毒蛋白时，在荧光免疫测定法中测量的异常病毒蛋白的量与根据蛋白质印迹估计的量相关。我们得出结论，该方法可用于检测组织样本中的异常病毒蛋白。 [参考：20]

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