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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Peptide separations and dissociation constants in nonaqueous capillary electrophoresis: Comparison of methanol and aqueous buffers.
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Peptide separations and dissociation constants in nonaqueous capillary electrophoresis: Comparison of methanol and aqueous buffers.

机译：非水毛细管电泳中的肽分离和解离常数：甲醇和水性缓冲液的比较。

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摘要

Nonaqueous capillary electrophoresis was evaluated for its potential to separate peptides in methanolic background electrolytes in comparison to aqueous-methanol (50% v/v) and water. Isomeric aspartyl dipeptides and Leu- and Met-enkephalin served as model compounds. pK(a) values were determined in the three solvent systems based on the apparent pH scale and in the case of methanol additionally based on the conventional pH scale. Changing from water to methanol led to an increase of the ionization constants describing the dissociation equilibria of the carboxyl group and the amino group, respectively. The pK(a) shift was more pronounced for the carboxylic acid function leading to a compression of the mobility-pH curve. As reported for aqueous buffers, efficient separations of the peptides were achieved in methanolic background electrolytes including the resolution of the diastereomers of the isomeric alpha- and beta-aspartyl dipeptides. In contrast to aqueous buffers, the separation of Leu- and Met-enkephalin could also be obtained in buffers in methanol at high pH.
机译：与含水甲醇（50％v / v）和水相比，评估了非水毛细管电泳分离甲醇背景电解质中肽的潜力。 Isomeric天冬氨酰二肽和亮氨酸和蛋氨酸脑啡肽用作模型化合物。在三种溶剂体系中，根据表观pH值确定pK（a）值，在甲醇的情况下，还根据常规pH值确定pK（a）值。从水变成甲醇导致电离常数的增加，分别描述了羧基和氨基的离解平衡。 pK（a）位移对于羧酸功能更为明显，导致迁移率-pH曲线压缩。如关于水性缓冲液的报道，在甲醇背景电解质中实现了肽的有效分离，包括拆分了异构的α-和β-天冬氨酰二肽的非对映异构体。与水性缓冲液相反，在高pH值的甲醇缓冲液中也可以分离亮氨酸和甲乙脑。

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