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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >CAPILLARY ELECTROPHORETIC ANALYSIS OF CYCLODEXTRINS AND DETERMINATION OF FORMATION CONSTANTS FOR INCLUSION COMPLEXES
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CAPILLARY ELECTROPHORETIC ANALYSIS OF CYCLODEXTRINS AND DETERMINATION OF FORMATION CONSTANTS FOR INCLUSION COMPLEXES

机译：环糊精的毛细管电泳分析和包合物的形成常数的测定

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摘要

Capillary zone electrophoresis (CZE) methods with indirect absorbance detection for analyzing mixtures of alpha-, beta-, and gamma-cyclodextrins (CDs) and their derivatives have been developed. Benzylamine, salicylic, sorbic, or 1-naphthylacetic acid (NAA) was utilized as background electrolyte (BGE) and absorbance provider. Separation of alpha-, beta-, and gamma-CD could be achieved in less than 18 min when the CZE was run in 2 mM NAA or 5 mM sorbate solution (pH 12.2) and detected by indirect absorbance at 222 or 254 nm, respectively. Mixtures of alpha- and beta-CDs, and dimethyl- and trimethyl-derivatives of beta-CD could also be analyzed by CZE, using 50 mM salicylic acid or benzylamine solution (pH 6.0) as BGE with indirect absorbance detection at 230 and 210 nm, respectively. CZE methods for determining the inclusion complex formation constants of various CDs for salicylic acid or benzylamine with either direct or indirect absorbance detection have also been developed. The formation constants of salicylate are in the range from ca. 8 +/- 0.3 mole(-1) for the complex with alpha-CD to ca. 99 +/- 2 molarity(-1) for the complex with methyl-beta-CD. The detection limits (determined at a signal-to-noise ratio of 3) for the NAA and the salicylate system are ca. 0.1 mM and 1 mM, respectively. [References: 15]
机译：已经开发了具有间接吸光度检测功能的毛细管区带电泳（CZE）方法，用于分析α-，β-和γ-环糊精（CD）及其衍生物的混合物。苄胺，水杨酸，山梨酸或1-萘乙酸（NAA）被用作背景电解质（BGE）和吸收剂。当CZE在2 mM NAA或5 mM山梨酸酯溶液（pH 12.2）中运行并分别通过222或254 nm处的间接吸光度检测时，可以在不到18分钟的时间内完成α-，β-和γ-CD的分离。 。也可以使用50 mM水杨酸或苄胺溶液（pH 6.0）作为BGE通过CZE分析α-和β-CD的混合物以及β-CD的二甲基和三甲基衍生物，并在230和210 nm处进行间接吸光度检测， 分别。还开发了用于通过直接或间接吸光度检测来确定水杨酸或苄胺的各种CD的包合物形成常数的CZE方法。水杨酸盐的形成常数在约1至约2的范围内。含α-CD的配合物约为8 +/- 0.3 mole（-1）。与甲基-β-CD的配合物的摩尔浓度为99 +/- 2（-1）。 NAA和水杨酸盐系统的检出限（以3的信噪比确定）为。分别为0.1 mM和1 mM。 [参考：15]

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