...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >HIGH PERFORMANCE CAPILLARY ELECTROPHORESIS METHOD TO CHARACTERIZE HEPARIN AND HEPARAN SULFATE DISACCHARIDES
【24h】

HIGH PERFORMANCE CAPILLARY ELECTROPHORESIS METHOD TO CHARACTERIZE HEPARIN AND HEPARAN SULFATE DISACCHARIDES

机译：高效毛细管电泳法表征肝素和肝素硫酸盐二糖

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A rapid, sensitive and accurate high-performance capillary electrophoresis method is described for the determination of the sulfation pattern of heparin and heparan sulfate disaccharides. The analysis, performed after enzymic degradation of the polysaccharides with heparinase and heparinases II and III in combination, yields highly UV-absorbing Delta-disaccharides. The separation is performed with reversed polarity using 15 mM phosphate buffer, pH 3.50. This method is superior to others since all known 12 disaccharides carrying N-acetylated, N-sulfated or unsubstituent glucosamine can be separated in a single run of 15 min. At the highest sensitivity the analysis consumes only a few femtograms of glycosaminoglycan and allows a determination of Delta-disaccharides at the attomole level. [References: 13]
机译：描述了一种快速，灵敏和准确的高性能毛细管电泳方法，用于测定肝素和硫酸乙酰肝素二糖的硫酸化模式。用肝素酶和肝素酶II和III组合酶降解多糖后进行的分析产生了高度吸收紫外线的Delta-二糖。使用pH 3.50的15 mM磷酸盐缓冲液，以反极性进行分离。该方法优于其他方法，因为所有已知的带有N-乙酰化，N-硫酸化或未取代的葡糖胺的12种二糖都可以在15分钟内完成分离。在最高灵敏度下，该分析仅消耗几克糖胺聚糖的飞克图，并可以测定attomole水平的Delta-二糖。 [参考：13]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号