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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >INDIRECT UV DETECTION OF CARBOHYDRATES IN CAPILLARY ZONE ELECTROPHORESIS BY USING TRYPTOPHAN AS A MARKER
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INDIRECT UV DETECTION OF CARBOHYDRATES IN CAPILLARY ZONE ELECTROPHORESIS BY USING TRYPTOPHAN AS A MARKER

机译：TRYPTOPHAN作为标记物间接间接检测毛细管区带电泳中的碳水化合物

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A rapid and sensitive method for the analysis of sugars was developed by capillary zone electrophoresis (CZE) with indirect UV detection. A mixture of nine mono- and oligosaccharides was separated in about 20 minutes on a fused silica capillary in a highly alkaline (pH > 12) background electrolyte (BGE). With tryptophan as the marker detected at 280 nm the femtomole level detection of sugars was possible. The system was optimized by studies on the buffer pH, the marker concentration, as well as the inner diameter of the capillary. The indirect detection of sugars was also performed with a cationic marker, N-benzylcinchonidium chloride (BCDC). However, the detection sensitivity was much lower in comparison to the tryptophan marker system. [References: 24]
机译：通过毛细管区带电泳（CZE）和间接UV检测，开发了一种快速，灵敏的糖分析方法。在高碱性（pH> 12）背景电解质（BGE）中，在熔融石英毛细管上约20分钟内分离出9种单糖和寡糖的混合物。用色氨酸作为在280 nm处检测到的标记物，可以检测糖的飞沫水平。通过研究缓冲液的pH，标记物浓度以及毛细管的内径来优化系统。糖的间接检测也可以使用阳离子标记物N-苄基金鸡氏氯化钠（BCDC）进行。但是，与色氨酸标记系统相比，检测灵敏度要低得多。 [参考：24]

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