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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Analytical potential of enzyme-coated capillary reactors in capillary zone electrophoresis
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Analytical potential of enzyme-coated capillary reactors in capillary zone electrophoresis

机译：酶包被的毛细管反应器在毛细管区带电泳中的分析潜力

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摘要

Enzymes immobilized on the inner surface of an electrophoretic capillary were used to increase sensitivity and resolution in capillary zone electrophoresis (CZE). Sensitivity is enhanced by inserting a piece of capillary containing the immobilized enzyme into the main capillary, located before the detector, in order to transform the analyte into a product with a higher absorptivity. This approach was used to determine ethanol. In order to improve resolution, capillary pieces containing immobilized enzymes were inserted at various strategic positions along the electrophoretic capillary. On reaching the enzyme, the analyte was converted into a product with a high electrophoretic mobility, the migration time for which was a function of the position of the enzyme reactor. This approach was applied to the separation and determination of acetaldehyde and pyruvate. Finally, the proposed method was validated with the determination of ethanol, acetaldehyde, and pyruvate in beer and wine samples. [References: 21]
机译：固定在电泳毛细管内表面的酶可用于提高毛细管区带电泳（CZE）的灵敏度和分辨率。通过将一块包含固定化酶的毛细管插入位于检测器之前的主毛细管中，以提高分析物的吸收率，从而提高灵敏度。该方法用于确定乙醇。为了提高分离度，将包含固定化酶的毛细管片段插入电泳毛细管的各个关键位置。到达酶后，分析物被转化为具有高电泳迁移率的产物，其迁移时间是酶反应器位置的函数。该方法用于乙醛和丙酮酸的分离和测定。最后，通过测定啤酒和葡萄酒样品中的乙醇，乙醛和丙酮酸，验证了该方法的有效性。 [参考：21]

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