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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Capillary electrophoresis of amphipathic alpha-helical peptide diastereomers
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Capillary electrophoresis of amphipathic alpha-helical peptide diastereomers

机译：两亲性α-螺旋肽非对映异构体的毛细管电泳

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摘要

We have made a rigorous assessment of the ability of capillary electrophoresis to resolve peptide diastereomers through its application to the separation of a series of synthetic 18-residue, amphipathic alpha-helical monomeric peptide analogues, where a single site in the centre of the hydrophobic face of the alpha-helix is substituted by 19 L- or D-amino acids. Such L- and D-peptide pairs have the same mass-to-charge ratio, amino acid sequence and intrinsic hydrophobicity, varying only in the stereochemistry of one residue. CE approaches assessed in their ability to separate diastereomeric, peptide pairs included capillary zone electrophoresis (uncoated capillary), micellar electrokinetic chromatography (uncoated capillary in the presence of 3-[(3-cholamido-propyl)dimethylammonio]-1-propanesulfonate, CHAPS), open-tubular capillary electrochromatography (C-8-coated capillary in the presence of 25% 2,2,2-trifluoroethanol (TFE) or 25% ethanol). Overall, the OT-CEC methods were the most effective at separating the most peptide pairs, particularly for those containing hydrophilic side chains. However, the MEKC approach proved most effective for separation of peptide pairs containing hydrophobic or aromatic side chains. [References: 36]
机译：我们已经通过将毛细管电泳应用于一系列合成的18残基，两亲性α-螺旋单体肽类似物的分离中，对毛细管电泳解决肽非对映异构体的能力进行了严格的评估，其中单个位点位于疏水面的中心α-螺旋的一部分被19个L-或D-氨基酸取代。这样的L-和D-肽对具有相同的质荷比，氨基酸序列和固有疏水性，仅在一个残基的立体化学上变化。 CE方法对非对映异构体，肽对的分离能力进行了评估，包括毛细管区带电泳（未涂覆的毛细管），胶束电动色谱法（在存在3-[（（3-胆酰胺基-丙基）二甲基铵基] -1-丙磺酸盐，CHAPS的情况下）未涂覆的毛细管，开管毛细管电色谱法（在25％2,2,2-三氟乙醇（TFE）或25％乙醇存在下，进行C-8涂层的毛细管）。总的来说，OT-CEC方法在分离大多数肽对中最为有效，尤其是对于那些含有亲水性侧链的肽。然而，事实证明，MEKC方法对于分离含有疏水或芳族侧链的肽对最为有效。 [参考：36]

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