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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >CHARACTERIZING ALLELIC PROTEINS FOR GENOME MAPPING IN MAIZE
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CHARACTERIZING ALLELIC PROTEINS FOR GENOME MAPPING IN MAIZE

机译：表征玉米中基因组映射的等位基因蛋白

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Mapping cDNA probes in order to construct genetic linkage maps is becoming a widespread strategy for genome analysis and gene isolation, particularly in cultivated plant species. Nevertheless, almost all cDNAs reveal two or more unlinked loci, making it difficult to identify the gene(s) actually expressed. In a highly polymorphic species, such as maize, two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) of proteins may circumvent this limitation. With the analysis of various segregating populations, we previously showed that the apparent position shifts of proteins on the 2-D gels are monogenic and codominant. In this paper we compared allelic proteins on the basis of their high performance liquid chromatography (HPLC) profile and partial amino acid sequences. In a sample of 20 position shifts, the allelic proteins appeared to be similar in all but one case, strongly suggesting that polymorphism of structural genes is involved. Thus 2-D PAGE could prove to be a useful tool for genome mapping: when a cDNA probe detects several loci, a position shift of the encoded protein will allow the identification of the gene translated in the organ considered. [References: 24]
机译：映射cDNA探针以构建遗传连锁图正在成为基因组分析和基因分离的一种广泛策略，尤其是在栽培植物物种中。然而，几乎所有的cDNA都揭示了两个或多个未连接的基因座，从而难以鉴定实际表达的基因。在高度多态的物种（例如玉米）中，蛋白质的二维聚丙烯酰胺凝胶电泳（2-D PAGE）可能会绕开此限制。通过对各种隔离种群的分析，我们以前表明了二维凝胶上蛋白质的表观位置偏移是单基因的，并且是显性的。在本文中，我们基于高效液相色谱（HPLC）图谱和部分氨基酸序列比较了等位基因蛋白。在20个位置偏移的样本中，除一种情况外，所有等位基因蛋白质似乎都相似，这强烈表明涉及结构基因的多态性。因此，2-D PAGE可能被证明是用于基因组作图的有用工具：当cDNA探针检测到多个基因座时，编码蛋白质的位置移动将允许鉴定所考虑器官中翻译的基因。 [参考：24]

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