...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Physicochemical characterization of phosphinic pseudopeptides by capillary zone electrophoresis in highly acidic background electrolytes
【24h】

Physicochemical characterization of phosphinic pseudopeptides by capillary zone electrophoresis in highly acidic background electrolytes

机译：高酸性背景电解质中毛细管区带电泳对次膦酸假肽的理化特性

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Phosphinic pseudopeptides (i.e., peptide isosteres with one peptide bond replaced by a phosphinic acid moiety) were analyzed and physicochemically characterized by capillary zone electrophoresis in the pH range of 1.1-3.2, employing phosphoric, phosphinic, oxalic and dichloroacetic acids as background electrolyte (BGE) constituents. The acid dissociation constant (pK(a)) of phosphinate group in phosphinic pseudopeptides and ionic mobilities of these analytes were determined from the pH dependence of their effective electrophoretic mobilities corrected to standard temperature and constant ionic strength of the BGEs. It was shown that these corrections are necessary whenever precise mobility data at very low pH are to be determined. Additionally, it was found that the ionic mobilities of the phosphinic pseudopeptides and pK(a) of their phosphinate group are affected by the BGE constituent used. The variability of migration behavior of the pseudopeptides can be attributed to their ion-pairing formation with the BGE components. [References: 27]
机译：使用磷酸，次膦酸，草酸和二氯乙酸作为背景电解质（BGE），分析膦酸假肽（即具有一个被膦酸部分取代的一个肽键的肽等排体），并通过在1.1-3.2的pH范围内的毛细管区带电泳进行理化表征。 ）成分。根据校正至标准温度的有效电泳迁移率的pH依赖性和BGE的恒定离子强度，可确定次要膦酸假肽中次膦酸酯基的次膦酸酯基的酸解离常数（pK（a））和离子迁移率。结果表明，每当要在非常低的pH下确定精确的迁移率数据时，这些校正都是必要的。另外，发现次膦酸酯假肽的离子迁移率及其次膦酸酯基团的pK（a）受所用BGE成分的影响。假肽迁移行为的可变性可归因于它们与BGE组分的离子配对形成。 [参考：27]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号