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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Surface modification of poly(methyl methacrylate) for improved adsorption of wall coating polymers for microchip electrophoresis
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Surface modification of poly(methyl methacrylate) for improved adsorption of wall coating polymers for microchip electrophoresis

机译：聚（甲基丙烯酸甲酯）的表面改性，可改善壁涂层聚合物的吸附性，以用于微芯片电泳

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摘要

The development of rapid and simple wall coating strategies for high-efficiency electrophoretic separation of DNA is of crucial importance for the successful implementation of miniaturized polymeric DNA analysis systems. In this report, we characterize and compare different methods for the chemical modification of poly(methyl methacrylate) (PMMA) surfaces for the application of wall coating polymers. PMMA surfaces coated with 40 mol% diethylacrylamide and 60 mol% dimethylacrylamide are compared to the PMMA surfaces first oxidized and then coated with hydroxypropylmethylcellulose or poly(vinyl alcohol) (PVA). PMMA oxidation was accomplished with UW ozone or an aqueous solution of HNO3 to yield hydrogen-bond donors for the spontaneous adsorption of the coating polymers. Contact angle measurements of UV/ozone exposed PMMA surfaces indicate increase in hydrophilicity, and polymer coated surfaces show a strong dependence on the coating polymer and the oxidation method. Fast and repeatable electrophoretic separations of a 10-base and 20-base DNA ladder were performed in PMMA micro CE devices. All analyses were completed in less than 10 min, resulting in the number of theoretical plates as high as 583 000 in a 7.7 cm long separation channel. The duration of UV/ozone treatment was found to have a considerable impact on separation performance. The microchips irradiated with UV for 10 min and coated with PVA as well as the microchips treated with HNO3 and coated with HPMC were found to have the best separation performance. These results demonstrate facile and robust methods for the surface modification of PMMA enabling low-cost single use devices for electrophoretic DNA separations.
机译：快速高效的DNA高效电泳分离壁涂策略的发展对于成功实施小型聚合DNA分析系统至关重要。在这份报告中，我们表征和比较了不同的化学改性聚甲基丙烯酸甲酯（PMMA）表面的方法，以用于墙面涂料聚合物。将涂覆有40 mol％的二乙基丙烯酰胺和60 mol％的二甲基丙烯酰胺的PMMA表面与先氧化然后涂有羟丙基甲基纤维素或聚乙烯醇（PVA）的PMMA表面进行比较。用UW臭氧或HNO3水溶液完成PMMA氧化，生成氢键供体，用于涂料聚合物的自发吸附。紫外线/臭氧暴露的PMMA表面的接触角测量表明亲水性增加，聚合物涂层表面显示出对涂层聚合物和氧化方法的强烈依赖性。在PMMA微型CE设备中对10个碱基和20个碱基的DNA梯子进行了快速且可重复的电泳分离。所有分析均在不到10分钟的时间内完成，因此在7.7厘米长的分离通道中，理论塔板数高达583000。发现UV /臭氧处理的持续时间对分离性能有相当大的影响。发现用紫外线辐照10分钟并用PVA涂覆的微芯片以及用HNO 3处理并用HPMC涂覆的微芯片具有最好的分离性能。这些结果表明，对PMMA进行表面修饰的方法简便而有效，可实现用于电泳DNA分离的低成本一次性设备。

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