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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Electrochromatographic behavior of silica monolithic capillaries of different skeleton sizes synthesized with a simplified and shortened sol-gel procedure
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Electrochromatographic behavior of silica monolithic capillaries of different skeleton sizes synthesized with a simplified and shortened sol-gel procedure

机译：通过简化和缩短的溶胶-凝胶程序合成的不同骨架尺寸的硅胶整体毛细管的电色谱行为

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摘要

Silica monolithic capillaries (SMCs) were synthesized by a sol-gel process. First, a simplification of the synthesis was proposed by replacing the calcination and the drying steps which can have tremendous effects on chromatographic and physical properties, by a single water or methanol 2 h washing step. The efficiency of such a washing step was demonstrated and the comparison of the chromatographic and electrochromatographic properties between calcined and washed SMCs has shown that such a modification did not impair retention, efficiency, and stability of the monolith. This simplified procedure was carried out to synthesize SMCs with two different skeleton sizes. These capillaries were evaluated in electrochromatography and present high efficiencies (H = 5 mu m) at least equal to the best ones reported in the literature. Furthermore, the influence of the skeleton size on the EOF of the second kind (EOF-2) was investigated with unmodified SMCs used under various experimental conditions including electrical field strength and buffer concentration. The ionic strength of the mobile phase and the applied electrical field that enable this EOF-2 were related to the size of the skeleton which was tuned by the synthesis conditions.
机译：二氧化硅整体毛细管（SMCs）是通过溶胶-凝胶法合成的。首先，提出了一种简化合成的方法，即通过一次水或甲醇洗涤2小时即可代替煅烧和干燥步骤，从而对色谱和物理性能产生巨大影响。证明了这种洗涤步骤的效率，并且煅烧和洗涤的SMC之间的色谱和电色谱性质的比较表明，这种修饰不会损害整料的保留，效率和稳定性。进行了此简化过程，以合成具有两种不同骨架尺寸的SMC。这些毛细管在电色谱中进行了评估，并显示出高效率（H = 5μm），至少等于文献中报道的最佳效率。此外，在包括电场强度和缓冲液浓度在内的各种实验条件下，使用未改性的SMC，研究了骨架尺寸对第二种EOF（EOF-2）的影响。使得该EOF-2的流动相的离子强度和施加的电场与通过合成条件调节的骨架尺寸有关。

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