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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Micromachined filter-chamber array with passive valves for biochemical assays on beads
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Micromachined filter-chamber array with passive valves for biochemical assays on beads

机译：带被动阀的微机械滤室阵列，用于珠子生化分析

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The filter-chamber array presented here enables a real-time parallel analysis of three different samples on beads in a volume of 3 nL, on a 1 cm(2) chip. The filter-chamber array is a system containing three filter-chambers, three passive valves at the inlet channels and a common outlet. The design enables parallel sample handling and time-controlled analysis. The device is microfabricated in silicon and sealed with a Pyrex lid to enable real-time analysis. Single nucleotide polymorphism analysis by using pyrose-quencing has successfully been performed in single filter-chamber devices. The passive valves consist of plasma-deposited octafluorocyclobutane and show a much higher resistance towards water and surface-active solutions than previous hydrophobic patches. The device is not sensitive to gas bubbles, clogging is rare and reversible, and the filter-chamber array is reusable. More complex (bio)chemical reactions on beads can be performed in the devices with passive valves than in the devices without valves. [References: 11]
机译：此处介绍的滤室阵列可对1 cm（2）芯片上3 nL体积的磁珠上的三个不同样品进行实时并行分析。滤室阵列是一个系统，包含三个滤室，入口通道上的三个被动阀和一个公共出口。该设计可实现并行样品处理和时间控制的分析。该设备采用硅微制造，并用耐热玻璃盖密封以实现实时分析。在单个滤室设备中已成功执行了通过使用吡咯测序的单核苷酸多态性分析。被动阀由等离子体沉积的八氟环丁烷组成，与以前的疏水性贴片相比，对水和表面活性溶液的耐受性高得多。该设备对气泡不敏感，堵塞很少且可逆，并且滤室阵列可重复使用。在具有被动阀的设备中，与在没有阀门的设备中相比，可在珠子上进行更复杂的生物化学反应。 [参考：11]

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