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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >PULSED ELECTROCHEMICAL DETECTION OF NONCHROMOPHORIC COMPOUNDS FOLLOWING CAPILLARY ELECTROPHORESIS
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PULSED ELECTROCHEMICAL DETECTION OF NONCHROMOPHORIC COMPOUNDS FOLLOWING CAPILLARY ELECTROPHORESIS

机译：毛细管电泳后脉冲色谱法检测非铬化合物

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Pulsed electrochemical detection (FED) following capillary electrophoresis (CE) has been applied to the direct detection of polar aliphatic compounds. These compounds typically have poor optical detection properties and are considered to be electroinactive under constant applied potentials. The voltammetric responses at microelectrodes of model compounds were studied under various buffers and pH conditions. The detection of unsubstituted carbohydrates requires highly alkaline conditions, whereas amine-containing compounds (e.g., glycopeptides, peptides, and amino acids) and thiocompounds can best be detected at mildly alkaline (i.e., pH 9.0) and mildly acidic (i.e., pH 5.5) conditions, respectively. In a comparative study of pulsed amperometric detection (PAD) and integrated pulsed amperometric detection (IPAD), IPAD is determined to be better suited to manage the large oxide-formation backgrounds, which accompany oxide-catalyzed detections of amine- and sulfur-containing compounds. IPAD results in more stable baselines, eliminates oxide-induced artifacts, and often yields lower limits of detection. Mass detection limits under optimal conditions are typically 10 fmole or less. This paper delineates FED waveform parameters for the optimal detection of underivatized nonchromophoric compounds of biological significance in various operating buffers and electrolytes. [References: 26]
机译：毛细管电泳（CE）之后的脉冲电化学检测（FED）已应用于极性脂族化合物的直接检测。这些化合物通常具有较差的光学检测性能，并被认为在恒定施加电势下是电惰性的。在各种缓冲液和pH条件下研究了模型化合物在微电极上的伏安响应。未取代碳水化合物的检测需要强碱性条件，而含胺化合物（例如，糖肽，肽和氨基酸）和硫化合物最好在弱碱性（即pH 9.0）和弱酸性（即pH 5.5）下检测。条件。在对脉冲安培检测（PAD）和集成脉冲安培检测（IPAD）的比较研究中，确定IPAD更适合管理大型氧化物形成背景，该背景伴随氧化物催化的胺和硫化合物的检测。 IPAD可使基线更稳定，消除氧化物引起的伪影，并且通常产生较低的检测限。最佳条件下的质量检测极限通常为10 fmole或更低。本文描述了FED波形参数，用于最佳检测在各种操作缓冲液和电解质中具有生物学意义的未衍生化非发色化合物。 [参考：26]

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