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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >CAPILLARY ELECTROPHORESIS AND ELECTROCHEMICAL DETECTION OF UNDERIVATIZED OLIGO- AND POLYSACCHARIDES WITH SURFACTANT-CONTROLLED ELECTROOSMOTIC FLOW
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CAPILLARY ELECTROPHORESIS AND ELECTROCHEMICAL DETECTION OF UNDERIVATIZED OLIGO- AND POLYSACCHARIDES WITH SURFACTANT-CONTROLLED ELECTROOSMOTIC FLOW

机译：表面活性剂控制的电渗流的毛细管电泳和电化学检测低价寡糖和多糖

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Complex polysaccharide mixtures were analyzed directly without derivatization by capillary zone electrophoresis in strongly alkaline solutions and electrochemical detection at a Cu electrode. The positively charged surfactant cetyltrimethylammonium bromide was included in the electrophoresis medium in order to reverse the electroosmotic flow and permit elution to be in order of increasing polysaccharide size. Carbohydrate samples analyzed by this approach included linear maltoses, enzymatically hydrolyzed starch, and commercially available dextrans of up to an average molecular weight of 18300. Detection by constant-potential oxidation at a Cu electrode was very sensitive, with detection limits for individual carbohydrates generally below the femtomole level. [References: 22]
机译：通过在强碱性溶液中通过毛细管区带电泳和在Cu电极上进行电化学检测，无需衍生化即可直接分析复杂的多糖混合物。电泳介质中包括带正电荷的表面活性剂十六烷基三甲基溴化铵，以使电渗流反向并允许洗脱，以增加多糖大小。通过这种方法分析的碳水化合物样品包括线性麦芽糖酶，酶水解淀粉和平均分子量高达18300的市售右旋糖酐。在铜电极上通过恒电位氧化检测非常敏感，单个碳水化合物的检测极限通常低于飞摩尔水平。 [参考：22]

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