Isolation of Multipotent Mesenchymal Stromal Cells from Cryopreserved Human Umbilical Cord Tissue

Romanov Yu. A.; Balashova E. E.; Volgina N. E.; Kabaeva N. V.; Dugina T. N.; Sukhikh G. T.

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Isolation of Multipotent Mesenchymal Stromal Cells from Cryopreserved Human Umbilical Cord Tissue

机译：从冷冻保存的人脐带组织中分离多能间质基质细胞

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Umbilical cord stroma is an easily available, convenient, and promising source of multipotent mesenchymal stromal cells for regenerative medicine. Cryogenic storage of umbilical cord tissue provides more possibilities for further isolation of multipotent mesenchymal stromal cells for autologous transplantation or scientific purposes. Here we developed a protocol for preparation of the whole umbilical cord tissue for cryogenic storage that in combination with the previously described modified method of isolation of multipotent mesenchymal stromal cells allowed us to isolate cells with high proliferative potential, typical phenotype, and preserved differentiation potencies.

机译：脐带基质是用于再生医学的多能间质基质细胞的一种容易获得，方便和有希望的来源。脐带组织的低温储存为自体移植或科研目的进一步分离多能间质基质细胞提供了更多可能性。在这里，我们开发了一种制备用于低温储存的整个脐带组织的方案，该方案与先前描述的分离多能间充质基质细胞的改良方法相结合，使我们能够分离出具有高增殖潜能，典型表型并保留分化潜能的细胞。

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《Bulletin of experimental biology and medicine》 |2016年第4期|共5页
作者
Romanov Yu. A.; Balashova E. E.; Volgina N. E.; Kabaeva N. V.; Dugina T. N.; Sukhikh G. T.;
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正文语种 eng
中图分类基础医学;
关键词
multipotent mesenchymal stromal cells; umbilical cord; cryopreservation; cell culture;

机译：多能间质基质细胞;脐带;冷冻保存;细胞培养;

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1. Isolation of Multipotent Mesenchymal Stromal Cells from Cryopreserved Human Umbilical Cord Tissue [J] . Romanov Yu. A., Balashova E. E., Volgina N. E., Bulletin of experimental biology and medicine . 2016,第4期

机译：从冷冻保存的人脐带组织中分离多能间质基质细胞
2. Optimized Protocol for Isolation of Multipotent Mesenchymal Stromal Cells from Human Umbilical Cord [J] . Romanov Yu. A., Balashova E. E., Volgina N. E., Bulletin of experimental biology and medicine . 2015,第1期

机译：优化方案，用于分离人脐带的多能分析基质细胞
3. Parameters that influence the isolation of multipotent mesenchymal stromal cells from human umbilical cord blood [J] . Attiyeh Vasaghi, Atefeh Dehghani, Zeinab Khademalhosseini, Hematology/Oncology and Stem Cell Therapy . 2013,第1期

机译：影响从人脐带血中分离多能间充质基质细胞的参数
4. SCALABLE EXPANSION OF HUMAN UMBILICAL CORD MATRIX- AND ADIPOSE TISSUE-DERIVED MESENCHYMAL STEM/STROMAL CELLS AND DERIVED EXOSOMES IN THE SINGLE-USE, VERTICAL-WHEEL BIOREACTOR SYSTEM USING A HUMAN PLATELET LYSATE CULTURE SUPPLEMENT [C] . Ana Fernandes-Platzgummer, Diogo de Sousa Pinto, Miguel de Almeida Fuzeta, Conference on advancing manufacture of cell and gene therapies . 2019

机译：单次使用垂直轮生物反应器系统中人类脐带基质和脂肪组织来源的间充质干细胞/基质细胞和衍生的外来体的可扩展性，使用人类血小板裂解液培养物
5. Tissue engineering the TMJ condyle using human umbilical cord mesenchymal stromal cells. [D] . Wang, Limin. 2008

机译：使用人脐带间充质基质细胞对TMJ dy进行组织工程。
6. Isolation and characterization of Wharton’s jelly-derived multipotent mesenchymal stromal cells obtained from bovine umbilical cord and maintained in a defined serum-free three-dimensional system [O] . Tereza C Cardoso, Heitor F Ferrari, Andrea F Garcia, 2012

机译：从牛脐带获得并保留在确定的无血清三维系统中的沃顿氏胶源多能间充质基质细胞的分离和鉴定
7. Parameters that influence the isolation of multipotent mesenchymal stromal cells from human umbilical cord blood [O] . Vasaghi Attiyeh, Dehghani Atefeh, Khademalhosseini Zeinab, 2013

机译：影响从人脐带血中分离多能间质基质细胞的参数

Isolation of Multipotent Mesenchymal Stromal Cells from Cryopreserved Human Umbilical Cord Tissue

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