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首页> 外文期刊>International Journal of Medicinal Mushrooms >Optimization of solid state culture conditions for the production of adenosine, cordycepin, and D-mannitol in fruiting bodies of medicinal caterpillar fungus Cordyceps militaris (L.:Fr.) link (ascomycetes).
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Optimization of solid state culture conditions for the production of adenosine, cordycepin, and D-mannitol in fruiting bodies of medicinal caterpillar fungus Cordyceps militaris (L.:Fr.) link (ascomycetes).

机译:固态毛虫真菌子实体 Cordyceps militaris (L.:Fr。)链接(子囊菌)中生产腺苷,虫草素和D-甘露醇的固态培养条件的优化。

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摘要

In general, Cordyceps sinensis is much more popular than C. militaris, though both species contain quite similar bioactive ingredients and exhibit medicinal activities. Many bioactive ingredients have been isolated from C. militaris, such as adenosine, cordycepin, D-mannitol, and exopolysaccharides. C. militaris is claimed to have extensive pharmacological properties, such as: anti-inflammatory; anti-fatigue; anti-bacterial; anti-diabetic; improve lung, liver, and kidney functions; to be beneficial for treating cancer as well as male and female sexual dysfunctions. C. militaris is fast gaining momentum for its so-called health benefits, and it is often used as a substitute for C. sinensis. In view of the growing popularity of C. militaris, nowadays C. militaris cultivation for stroma is also done. There is a great diversity of compounds from different strains of Cordyceps and different artificially cultivated products. This study is to determine the optimum culture parameters integrated with substrate of choice to bring the indoor-cultivated C. militaris to a higher and more consistent level of quality. To achieve the above objective, the resultant products after growth were analyzed for adenosine, cordycepin, and D-mannitol using the high-performance liquid chromatography method. The optimum culture condition to produce a high level of adenosine is by using millet as solid substrate. It must be cultivated in the dark for the first 7 days and harvested on day 40. The optimum culture condition to produce a high level of cordycepin is by using soybean as solid substrate. It must be cultivated in the dark for the first 14 days and harvested on day 50. While a high level of D-mannitol is achieved with millet as the solid substrate. It must be kept in the dark for the first 7 days and harvested on day 50. The adenosine level decreased and cordycepin increased from day 40 of culture to day 50 generally.
机译:通常,冬虫夏草比 C更受欢迎。尽管这两种物种都具有非常相似的生物活性成分并具有药用活性。已从iC中分离出许多生物活性成分。 mil,例如腺苷,虫草素,D-甘露醇和胞外多糖。 C。据称草具有广泛的药理特性,例如:抗炎药;抗疲劳;抗菌;抗糖尿病改善肺,肝和肾功能;对治疗癌症以及男性和女性性功能障碍有益。 C。 militaris 因其所谓的健康益处而迅速获得发展势头,它通常被用作 C的替代品。中华。鉴于C的日益普及。 militaris ,如今是C。还进行了军方间质的培养。来自虫草的不同菌株和不同的人工栽培产品的化合物种类繁多。这项研究是为了确定最佳的培养参数,并结合选择的底物以带来室内培养的iC。军事水平更高,更稳定的水平。为了实现上述目的,使用高效液相色谱法分析了生长后所得产物的腺苷,虫草素和D-甘露醇。产生高水平腺苷的最佳培养条件是使用小米作为固体底物。必须在前7天在黑暗中种植,并在第40天收获。要生产高水平的虫草素,最佳的培养条件是使用大豆作为固体基质。在开始的14天必须在黑暗中种植,并在第50天收获。以小米为固体底物可实现高水平的D-甘露醇。从培养的第40天到第50天,必须将其在前7天避光保存,并在第50天收获。腺苷水平降低而虫草素升高。

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