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首页> 外文期刊>International Journal of Medicinal Mushrooms >Chemical screening and identification of high cordycepin containing cultured isolate(s) of medicinal Chinese caterpillar mushroom, Ophiocordyceps sinensis (Berk.) G.H. Sung et al.
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Chemical screening and identification of high cordycepin containing cultured isolate(s) of medicinal Chinese caterpillar mushroom, Ophiocordyceps sinensis (Berk.) G.H. Sung et al.

机译:含有高虫草素的药用冬虫夏草(i.Ophiocordyceps sinensis (Berk。)G.H. Sung等。

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摘要

Forty isolates of Ophiocordyceps sinensis collected from Himalayan alpine meadows of Uttarakhand, India, and cultivated on Jhangora (Echinochloa crusgalli) grains were screened to identify the isolate(s) of high cordycepin content. The cultured mycelia were extracted with 50% methanol-chloroform and analyzed by HPTLC using chloroform:methanol (6:1 v/v) as mobile phase and densitometry scanning at 263 nm. Cordycepin varied from 0.002% to 0.029% was detected in twenty-one isolates. Compared to natural O. sinensis (0.004%, 0.006%), cordycepin was determined to be enhanced in twelve cultured samples.
机译:从印度Uttarakhand喜马拉雅高山草甸上采集并种植在贾汉拉( Echinochloa crusgalli )谷物上的40种中华麦冬菌菌株进行了筛选,以鉴定高虫草素的菌株。内容。用50%甲醇-氯仿提取培养的菌丝体,并使用氯仿:甲醇(6∶1v / v)作为流动相并通过263nm的光密度测定法进行HPTLC分析。在21个菌株中检测到的虫草素含量从0.002%到0.029%不等。与天然iO相比。中华((0.004%,0.006%),虫草素在十二个培养样品中被确定增强。

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