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首页> 外文期刊>EMBO reports >Nuclear transit of the RNA-binding protein She2 is required for translational control of localized ASH1 mRNA
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Nuclear transit of the RNA-binding protein She2 is required for translational control of localized ASH1 mRNA

机译：RNA结合蛋白She2的核转运是本地化ASH1 mRNA的翻译控制所必需的

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摘要

Cytoplasmic localization and localized translation of messenger RNAs contribute to asymmetrical protein distribution. Recognition of localized mRNAs by RNA-binding proteins can occur in the cytoplasm or, alternatively, co- or post-transcriptionally in the nucleus. In budding yeast, mRNAs destined for localization are bound by the She2 protein before their nuclear export. Here, we show that a specific transcript, known as ASH1 mRNA, and She2 localize specifically to the nucleolus when their nuclear export is blocked. Nucleolar She2 localization is enhanced in a She2 mutant that cannot bind to RNA. A fusion protein of the amino terminus of She3 and She2 (She3N-She2) fails to enter the nucleus, but does not impair ASH1 mRNA localization. Instead, these cells fail to distribute Ash1 protein asymmetrically, which is caused by a defective translational control of ASH1 mRNA. Our results indicate that the nucleolar transit of RNA-binding proteins such as She2 is necessary for the correct assembly of translationally silenced localizing messenger ribonucleoproteins.
机译：信使RNA的细胞质定位和局部翻译有助于蛋白质的不对称分布。 RNA结合蛋白对局部mRNA的识别可以发生在细胞质中，也可以发生在细胞核的共转录或转录后。在发芽酵母中，预定用于定位的mRNA在其核输出之前被She2蛋白结合。在这里，我们显示了一个特定的转录本，称为ASH1 mRNA和She2，当它们的核输出被阻滞时，它们专门定位在核仁上。在无法结合RNA的She2突变体中，核仁She2定位增强。 She3和She2的氨基末端融合蛋白（She3N-She2）不能进入细胞核，但不会损害ASH1 mRNA的定位。而是，这些细胞无法不对称地分配Ash1蛋白，这是由于ASH1 mRNA的翻译控制缺陷所致。我们的结果表明，RNA结合蛋白如She2的核仁转运对于正确组装翻译沉默的信使核糖核蛋白是必需的。

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