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首页> 外文期刊>Electroanalysis >Enhanced sensivity of a first generation glucose amperometric biosensor based on fibrinogen-precoating to carbon paste electrode
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Enhanced sensivity of a first generation glucose amperometric biosensor based on fibrinogen-precoating to carbon paste electrode

机译：增强的基于纤维蛋白原预涂的第一代葡萄糖安培生物传感器对碳糊电极的敏感性

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摘要

The performance of a first generation glucose amperometric biosensor was tested in the presence of immobilized fibrinogen (Fbg). Adsorption of the plasmatic protein was achieved at the surface of a carbon paste electrode by two different procedures. In the first, glucose oxidase (GOD) was adsorbed prior to Fbg; in the second, Fbg was adsorbed prior to GOD. Relative to surfaces with no Fbg, Fbg-coated surfaces exhibited comparable linearity and sensivity, except when the protein was coated prior to GOD. The latter sensors showed a five-fold gain in sensivity in extending the adsorption time of GOD to 60 min. This performance was essentially attributed to improved spatial conformation since, in this case, the amount of GOD was only 30 % higher, as assessed by means of ELISA tests. [References: 11]
机译：在固定化纤维蛋白原（Fbg）的存在下测试了第一代葡萄糖安培生物传感器的性能。通过两种不同的方法在碳糊电极的表面上实现了血浆蛋白的吸附。首先，在Fbg之前先吸附葡萄糖氧化酶（GOD）。在第二步中，Fbg在GOD之前被吸附。相对于没有Fbg的表面，Fbg包被的表面表现出可比的线性度和敏感性，但在GOD之前将蛋白包被时除外。后一种传感器在将GOD的吸附时间延长至60分钟时显示出五倍的灵敏度提高。该性能基本上归因于空间构象的改善，因为在这种情况下，通过ELISA试验评估，GOD的含量仅高30％。 [参考：11]

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