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Impedimetric Detection of DNA Damage with the Sensor Based on Silver Nanoparticles and Neutral Red

机译：基于银纳米粒子和中性红的传感器阻抗检测DNA损伤

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摘要

Novel electrochemical DNA-sensor based on glassy carbon electrode (GCE) modified with Ag nanoparticles, Neutral red covalently attached to its surface and native DNA adsorbed on modifier coating was developed for the estimation of DNA damage on example of model system based on Fenton reagent. As was shown, the oxidation process resulted in synchronous increase of electron transfer resistance and capacitance measured by electrochemical impedance spectroscopy (EIS). The contribution of each sensor component on the signal was specified and sensitivity estimated against similar surface coatings. The shift of EIS parameters was found to be higher than that of similar biosensors reported. The DNA sensor was tested on the estimation of antioxidant capacity of green tea infusions again the results of coulometric titration with electrogenerated bromine.
机译：以基于Fenton试剂的模型系统为例，开发了一种基于银纳米粒子修饰的玻碳电极（GCE），共价键附着于其表面的中性红和吸附在修饰剂涂层上的天然DNA的新型电化学DNA传感器，以估计DNA的损伤。如图所示，氧化过程导致通过电化学阻抗谱（EIS）测量的电子转移电阻和电容的同步增加。确定了每个传感器组件对信号的贡献，并针对相似的表面涂层估算了灵敏度。发现EIS参数的偏移高于所报道的类似生物传感器的偏移。对DNA传感器进行了测试，以评估绿茶浸出液的抗氧化能力，再次用电生成的溴进行库仑滴定的结果。

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