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Galactose Oxidase/Prussian Blue Based Biosensors

机译:基于半乳糖氧化酶/普鲁士蓝的生物传感器

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This paper describes the development of an amperometric biosensor based on galactose oxidase (GAOx) immobilization within a laponite clay film deposited on Carbon Screen-Printed Electrodes modified by electrodeposited Prussian Blue and coated with poly-(O-phenylenediamine) (PPD/PB/CSPEs). Amperometric performances of GAOx@laponite/PPD/PB/CSPEs bioelectrodes were determined using several GAOx substrates. Using these modified electrodes the reduction of enzymatically generated hydrogen peroxide was performed at -0.2V vs. Ag-AgCl. In an initial attempt, E.Coli transketolase activity on its immobilized form was followed using a bienzymatic GAOx-TK biosensor.
机译:本文介绍了一种基于半乳糖氧化酶(GAOx)固定在沉积在电沉积普鲁士蓝修饰的碳丝网印刷电极上并涂有聚(O-苯二胺)(PPD / PB / CSPEs)的碳丝网印刷电极上的半乳糖氧化粘土膜上的电流型生物传感器的开发)。使用几种GAOx底物测定了GAOx @ laponite / PPD / PB / CSPEs生物电极的安培性能。使用这些修饰的电极,相对于Ag-AgCl,在-0.2V下进行了酶促生成的过氧化氢的还原。在最初的尝试中,使用双酶GAOx-TK生物传感器追踪固定化形式的大肠杆菌转酮醇酶活性。

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