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Electrochemical and UV-Vis spectroscopic measurements of nitric oxide in fibroblasts and astrocytes

机译：电化学和紫外可见光谱法测量成纤维细胞和星形胶质细胞中一氧化氮

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Two complementary analytical methods were applied to measure nitric oxide production in two cell cultures of fibroblasts and astrocytes: W-vis spectroscopic assay of nitrites based on Griess reaction and direct, electrochemical determination of nitric oxide with use of a 5 mm diameter disk porphyrinic sensor. The nitrite measurement was used in order to get total quantitative information on NO production in a specified time. Application of an electrochemical sensor allowed observation of the dynamics and stabilization of nitric oxide production in real time, in the vicinity of a culture of stimulated cells. In experiments, cell suspension was placed just above the sensor surface or cells were grown directly on the modified electrode. Current curves were recorded after base current stabilization and injection of L-arginine the last remaining cosubstrate in the system. Collected data enabled one to estimate the local concentration of nitric oxide produced in the culture of fibroblasts to be 70 nM. [References: 22]
机译：应用了两种互补的分析方法来测量成纤维细胞和星形胶质细胞的两种细胞培养物中一氧化氮的产生：基于Griess反应的亚硝酸盐的W-vis光谱分析和使用直径5 mm的圆盘卟啉传感器直接电化学测定一氧化氮。使用亚硝酸盐测量是为了获得指定时间内NO产生的全部定量信息。电化学传感器的应用允许在刺激的细胞培养物附近实时观察一氧化氮产生的动力学和稳定性。在实验中，将细胞悬液放置在传感器表面上方，或者将细胞直接在修饰的电极上生长。在基本电流稳定并注入L-精氨酸后，记录电流曲线，该系统是系统中最后剩余的共底物。收集的数据使人们能够估计成纤维细胞培养物中产生的一氧化氮的局部浓度为70 nM。 [参考：22]

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