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首页> 外文期刊>Electroanalysis >Design of a multilayer cholesterol amperometric biosensor for preparation and use in flow systems
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Design of a multilayer cholesterol amperometric biosensor for preparation and use in flow systems

机译：用于流量系统的制备和使用的多层胆固醇安培生物传感器的设计

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A cholesterol amperometric biosensor based on a multilayer configuration with an initial platinized layer electrodeposited on the electrode surface and two sequentially electrosynthesized polymer layers was developed. The polymer bilayer was formed by an inner polypyrrole layer, in which cholesterol oxidase was entrapped and an outer layer of poly(o-phenylenediamine). All biosensor preparation steps and the determination of the substrate were carried out in a Row system, which permitted acceptable reproducibility of the fabrication process of each biosensor. The resulting biosensor, with a detection process based on the electrochemical oxidation at +0.5 V(vs. Ag/AgCl) of the enzymatically produced hydrogen peroxide, displayed high efficacy in the signal generation process (above 97 %) and had a stable response period of approximately one month. The presence of the two polymer layers had no incidence on the response time (t(95) = 8 s) and sensitivity and the detection limit are 62 nA mM(-1) and 12 muM, respectively The characteristics of the biosensor were compared to those of other biosensors with a more simple configuration, which confirmed the considerable advantages of the biosensor developed. The combined permselective properties of the two polymer layers permitted satisfactory determination of the cholesterol content in reference synthetic serum samples. [References: 35]
机译：开发了一种基于多层结构的胆固醇安培生物传感器，该多层结构具有在电极表面上电沉积的初始铂化层和两个顺序电合成的聚合物层。聚合物双层是由内包有胆固醇氧化酶的内聚吡咯层和聚（邻苯二胺）的外层形成的。所有生物传感器的制备步骤和底物的测定均在Row系统中进行，这使每个生物传感器的制造过程具有可接受的可重复性。所得生物传感器具有基于酶促产生的过氧化氢在+0.5 V（vs。Ag / AgCl）的电化学氧化下的检测过程，在信号产生过程中显示出较高的效率（97％以上），并且具有稳定的响应时间大约一个月。这两个聚合物层的存在与响应时间无关（t（95）= 8 s），灵敏度和检测极限分别为62 nA mM（-1）和12μM。具有更简单配置的其他生物传感器的传感器，证实了所开发生物传感器的显着优势。这两个聚合物层的组合的选择性渗透特性使得可以令人满意地确定参考合成血清样品中的胆固醇含量。 [参考：35]

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