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首页> 外文期刊>Electroanalysis >Streptavidin-polypyrrole Film as Platform for Biotinylated Redox Probe Immobilization for Electrochemical Immunosensor Application
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Streptavidin-polypyrrole Film as Platform for Biotinylated Redox Probe Immobilization for Electrochemical Immunosensor Application

机译：链霉亲和素-聚吡咯膜作为生物素化氧化还原探针固定化平台的电化学免疫传感器应用

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摘要

A platform for construction of multi-array electrochemical immunosensors was developed in which the measurement could be performed by various targets depending on the potential ranges of electrochemical detection. This was demonstrated by the immobilization of biotinylated redox molecules such as viologen and ferrocene, on modified polypyrrole-streptavidin film. The polypyrrole film was modified by streptavidin layer, which serves for both the attachment of biotinylated redox marker and biotinylated antibody. Such biologically modified surface exhibits variation of electrochemical response of redox molecule at various potential ranges after antibody/antigen interaction. The measurement of redox signal of viologen and ferrocene was analyzed by differential pulse voltammetry (DPV) method and underlines a variation of redox properties upon the antigen interaction. Viologen immobilized on polypyrrole /streptavidin surface shows a redox signal at -1.2V versus Ag/AgCl corresponding to the reduction of dicationic viologen to neutral viologen within 2 electrons process. Immobilized biotinylated ferrocene exhibits redox signal at 0.4V versus Ag/AgCl. We demonstrate that when the biolayer bearing antibody is in contact with antigen, the redox signal of ferrocene or viologen was modified. The variation of current signal is directly related to the concentration of antigen in solution. A detection limit ofpg.mL(-1) was obtained demonstrating the sensitivity of such electrochemical immunosensor. The modified streptavidin polypyrrole surface bearing various biotinylated redox markers is a proof of concept for multi-array electrochemical detection system of complex sample.
机译：开发了用于构建多阵列电化学免疫传感器的平台，其中可以根据电化学检测的潜在范围，通过各种目标进行测量。生物素化的氧化还原分子（如紫精和二茂铁）固定在改性的聚吡咯-链霉亲和素薄膜上可以证明这一点。聚吡咯膜被抗生蛋白链菌素层修饰，其既用于生物素化的氧化还原标记物的附着，又用于生物素化的抗体的附着。抗体/抗原相互作用后，这种经过生物修饰的表面在各种电势范围内表现出氧化还原分子的电化学响应变化。通过差分脉冲伏安法（DPV）分析了紫精和二茂铁的氧化还原信号的测量结果，并强调了抗原相互作用后氧化还原特性的变化。固定在聚吡咯/链霉亲和素表面上的紫精相对于Ag / AgCl在-1.2V处显示氧化还原信号，这对应于在两个电子过程中离子型紫精还原为中性紫精。固定化的生物素化的二茂铁相对于Ag / AgCl在0.4V时显示出氧化还原信号。我们证明当带有抗体的生物层与抗原接触时，二茂铁或紫精的氧化还原信号被修饰。电流信号的变化与溶液中抗原的浓度直接相关。获得了pg.mL（-1）的检测限，证明了这种电化学免疫传感器的灵敏度。修饰的抗生蛋白链菌素聚吡咯表面带有各种生物素化的氧化还原标记，是复杂样品多阵列电化学检测系统的概念验证。

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