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首页> 外文期刊>Electroanalysis >Characterization of a glutamate biosensor based on a novel glutamate oxidase integrated into a redox hydrogel
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Characterization of a glutamate biosensor based on a novel glutamate oxidase integrated into a redox hydrogel

机译：基于整合到氧化还原水凝胶中的新型谷氨酸氧化酶的谷氨酸生物传感器的表征

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A newly isolated and purified glutamate oxidase was used to construct amperometric biosensors for glutamate monitoring. The enzyme-producing microorganism belongs to the genus of Streptomyces - Streptomyces sp. Z-11-6 - and is capable of producing extracellular L-glutamate oxidase. The microorganism was obtained by mutagenesis with HNo2 from a parent strain isolated from soil of Lypetskaya region, Russia, followed by conventional screening methods. The developed biosensor is based on a coupled enzyme system (glutamate oxidase and horseradish peroxidase), the enzymes being crosslinked to a redox polymer (PVI19-dmeOs) using poly(ethylene glycol) diglycidyl ether as crosslinker. ne characteristics of the obtained biosensors were compared with those obtained for similarly constructed electrodes based on a commercially available glutamate oxidase from Yamasa Corp., Japan. The biosensors were operated at - 50 mV (vs. Ag/AgCl 0.1 M KCl) in a flow injection system. Special attention has been focused on the selectivity of the biosensors, evaluating their responses in the presence of several potentially interfering substances, possibly present in brain microdialysates (ascorbic acid, aspartate, cysteine, dopamine, DOPAC, GABA, glutamine, glycine, 5-HIAA, HMPG, HVA, serotonin, tryptophan, tyrosine and uric acid). The biosensors based on the two enzymes displayed similar bioanalytical characteristics but different selectivity patterns. [References: 39]
机译：新分离和纯化的谷氨酸氧化酶用于构建用于谷氨酸监测的安培生物传感器。产生酶的微生物属于链霉菌属-链霉菌属。 Z-11-6-并能够产生细胞外L-谷氨酸氧化酶。该微生物是通过用HNo2诱变从分离自俄罗斯Lypetskaya地区土壤的亲本菌株中获得的，然后进行常规筛选方法。研发的生物传感器基于偶联的酶系统（谷氨酸氧化酶和辣根过氧化物酶），这些酶使用聚（乙二醇）二缩水甘油醚作为交联剂交联到氧化还原聚合物（PVI19-dmeOs）上。将获得的生物传感器的特征与从日本山崎公司（Yamasa Corp.，Japan）商购的基于谷氨酸氧化酶的相似构造的电极获得的特征进行比较。生物传感器在流动注射系统中在-50 mV（vs。Ag / AgCl 0.1 M KCl）下运行。特别关注的是生物传感器的选择性，评估它们在几种潜在干扰物质（可能存在于脑微透析液（抗坏血酸，天冬氨酸，半胱氨酸，多巴胺，多巴胺，DOPAC，GABA，谷氨酰胺，甘氨酸，5-HIAA）中的存在下的反应，HMPG，HVA，5-羟色胺，色氨酸，酪氨酸和尿酸）。基于这两种酶的生物传感器显示出相似的生物分析特征，但选择性模式却不同。 [参考：39]

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