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首页> 外文期刊>Electroanalysis >Development of a multiresidue liquid chromatography method with electrochemical detection (HPLC-ECD) for the determination of macrolide antibiotics
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Development of a multiresidue liquid chromatography method with electrochemical detection (HPLC-ECD) for the determination of macrolide antibiotics

机译:电化学检测多残留液相色谱法(HPLC-ECD)用于大环内酯类抗生素测定的开发

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摘要

A multiresidue high performance liquid chromatography procedure with electrochemical detection (amperometric) (HPLC-ECD) using a C18 narrow bore column was fully optimized for the separation of a standard mixture of macrolides. Up to five macrolides, josamycin (JOS), kitasamycin (KIT), rosamicin (ROS) roxithromycin (ROX) and oleandomycin (OLE), the latter being selected as internal standard for the quantification, were properly separated using an isocratic elution mode. The influence of classical chromatographic and instrumental parameters was investigated in order to optimize them before the calibration assay. The method was succesfully applied to the determination of ROS and ROX in human urine. [References: 24]
机译:使用C18窄孔色谱柱的带有电化学检测(安培法)(HPLC-ECD)的多残留高效液相色谱程序已完全优化,可用于大环内酯化合物的标准混合物的分离。使用等度洗脱模式正确分离了多达5种大环内酯类药物,包括乔沙霉素(JOS),kitasamycin(KIT),rosamicin(ROS),罗红霉素(ROX)和oleandomycin(OLE),后者被选作定量内标。为了在校准分析之前优化它们,研究了经典色谱和仪器参数的影响。该方法成功应用于人尿中ROS和ROX的测定。 [参考:24]

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