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首页> 外文期刊>Electroanalysis >An amperometric biosensor based on the coimmobilization of horseradish peroxidase and methylene blue on a carbon nanotubes modified electrode
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An amperometric biosensor based on the coimmobilization of horseradish peroxidase and methylene blue on a carbon nanotubes modified electrode

机译：基于辣根过氧化物酶和亚甲基蓝共固定在碳纳米管修饰电极上的安培生物传感器

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A novel hydrogen peroxide biosensor has been constructed based on the characteristics of the carbon nanotube. The multiwall carbon nanotube (MWNT) was used as a coimmobilization matrix to incorporate horseradish peroxidase (HRP) and electron transfer mediator methylene blue (MB) onto a glassy carbon electrode surface. Cyclic voltammetry and amperometric measurements were employed to demonstrate the feasibility of methylene blue as an electron carrier between the immobilized peroxidase and the surface of glassy carbon electrode. The amperometric response of this resulting biosensor to H2O2 shows a linear relation in the range from 4 muM to 2 mM. The detection limit was 1 muM when the signal to noise ratio is 3. The presence of dopamine and ascorbic acid hardly affects the sensitive determination of H2O2. This biosensor also possesses very good stability and reproducibility. [References: 33]
机译：基于碳纳米管的特性，构建了一种新型的过氧化氢生物传感器。将多壁碳纳米管（MWNT）用作共固定基质，以将辣根过氧化物酶（HRP）和电子传递介体亚甲基蓝（MB）掺入玻璃碳电极表面。循环伏安法和安培法测量被用来证明亚甲基蓝作为固定化的过氧化物酶和玻璃碳电极表面之间电子载体的可行性。该所得生物传感器对H2O2的安培响应显示出从4μM到2 mM的线性关系。当信噪比为3时，检测限为1μM。多巴胺和抗坏血酸的存在几乎不会影响H2O2的灵敏测定。该生物传感器还具有非常好的稳定性和可重复性。 [参考：33]

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