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首页> 外文期刊>Electroanalysis >Amperometric detection of glucose with glucose oxidase absorbed on porous nanocrystalline TiO2 film
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Amperometric detection of glucose with glucose oxidase absorbed on porous nanocrystalline TiO2 film

机译：吸附在多孔纳米TiO2薄膜上的葡萄糖氧化酶用于安培检测葡萄糖

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Porous nanocrystalline TiO2 may be a promising material for enzyme immobilization owing to its high biocompatiability and large specific surface area of nanosized particles. We exploited a novel route to synthesize nanosized TiO2 particles with TiCl4 as precursor. With this method high purity of the oxide product can be obtained and the resulted particles were in the size range at about 5 nm. The enzyme loading with such material was 13 % (w/w, GOD/TiO2). The interaction between the enzyme and TiO2 matrix was investigated by IR and Raman, as the main characteristic peaks of native GOD could be found in TiO2-GOD at almost the same positions, it implied that the immobilization process did little harm to the structure of GOD. The nanocrystalline TiO2-GOD based sensor was fabricated. Amperometric detection of glucose with such an unmediated sensor at 0.7 V (vs. SCE) resulted in a rapid stable and linear response in the concentration range of 0-3 mM with apparent Michaelis-Menten constant K-M(app) of 6.08 mM, indicating that the immobilized GOD on nanosized TiO2 matrix retained its native activity. [References: 14]
机译：多孔纳米晶体TiO2由于其高生物相容性和纳米颗粒的大比表面积，可能是酶固定化的有前途的材料。我们开发了一种以TiCl4为前体合成纳米TiO2颗粒的新途径。用这种方法可以获得氧化物产物的高纯度，所得颗粒的尺寸范围为约5nm。用这种材料负载的酶为13％（w / w，GOD / TiO 2）。通过红外和拉曼光谱研究了酶与TiO2基质之间的相互作用，因为在几乎相同的位置可以在TiO2-GOD中发现天然GOD的主要特征峰，这表明固定化过程对GOD的结构影响很小。 。制备了纳米晶TiO2-GOD基传感器。在0.7 V（vs. SCE）下使用这种无介导的传感器对葡萄糖进行安培检测可以在0-3 mM的浓度范围内实现快速稳定且线性的响应，表观Michaelis-Menten常数KM（app）为6.08 mM，这表明固定在纳米TiO2基质上的GOD保留了其天然活性。 [参考：14]

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