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EDTA determination by urease-based inhibition biosensor

机译:基于脲酶的抑制生物传感器测定EDTA

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Urease immobilized into poly(vinylpyridine-co-styrene) film on the surface of ion sensitive field effect transistor (ISFET) has been used for determination of chelating agents (sequestrants). These species, particularly EDTA, restored catalytic activity of the enzyme if it was inhibited with cupric ions. Enzyme immobilization into a positively charged polymer seems to cause an increase of the EDTA restoration effect because of anion accumulation in the polymeric matrix. The resulting biosensor can measure EDTA concentration in the range of 5-500 ppm. The influence of other sequestrants (citrate and phosphate) were also examined. [References: 11]
机译:固定在离子敏感场效应晶体管(ISFET)表面的聚(乙烯基吡啶-共-苯乙烯)薄膜中的脲酶已用于测定螯合剂(螯合剂)。这些物质,特别是EDTA,如果被铜离子抑制,可恢复酶的催化活性。酶固定在带正电的聚合物中似乎会引起EDTA还原效果的增强,因为阴离子在聚合物基质中积累。所得的生物传感器可以测量5-500 ppm范围内的EDTA浓度。还研究了其他螯合剂(柠檬酸盐和磷酸盐)的影响。 [参考:11]

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