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首页> 外文期刊>Electroanalysis >Immobilized cytochrome c sensor in organic/aqueous media for the characterization of hydrophilic and hydrophobic antioxidants
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Immobilized cytochrome c sensor in organic/aqueous media for the characterization of hydrophilic and hydrophobic antioxidants

机译：固定在有机/水性介质中的细胞色素c传感器，用于表征亲水和疏水抗氧化剂

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A method for the characterization of antioxidants is introduced, which allows the measurement of pure hydrophilic and hydrophobic substances as well as complex cosmetic creams. The sensor is based on cytochrome c covalently immobilized on a gold wire electrode working in mixtures of phosphate buffer and organic solvents. It is combined with a superoxide generating enzyme system. The decrease of the superoxide concentration in the test solution by the added antioxidants is detected and used for the quantification of their antioxidative efficiency. Electrochemical proper-ties of immobilized cytochrome c, such as formal potential and heterogeneous electron transfer rate constant, have been investigated in mixtures of aqueous buffer and DMSO, methanol, butanediol, and THE The maximum organic solvent content for quasi-reversible electrode behavior was correlated to spectroscopic measurements. The activity of the radical producing enzyme in such media was determined and the radical generation characterized. The antioxidative proper-ties of pure substance such as ascorbic acid and Biochanin A as well as of five anti-ageing cosmetic creams were studied. This showed also the influence of matrix composition on the efficiency of antioxidative supplements. [References: 37]
机译：介绍了一种表征抗氧化剂的方法，该方法可以测量纯亲水性和疏水性物质以及复杂的美容霜。该传感器基于共价固定在金丝电极上的细胞色素c，该电极在磷酸盐缓冲液和有机溶剂的混合物中工作。它与产生超氧化物的酶系统结合。检测到通过添加抗氧化剂所导致的测试溶液中超氧化物浓度的降低，并将其用于定量其抗氧化效率。已研究了在水性缓冲液和DMSO，甲醇，丁二醇和四氢呋喃的混合物中固定化细胞色素c的电化学性质，例如形式势和异质电子转移速率常数，与准可逆电极行为的最大有机溶剂含量相关光谱测量。测定了在这种培养基中产生自由基的酶的活性并表征了自由基的产生。研究了抗坏血酸和Biochanin A等纯物质以及五种抗衰老美容霜的抗氧化性能。这也表明基质组成对抗氧化补充剂效率的影响。 [参考：37]

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