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首页> 外文期刊>Electroanalysis >Direct electrochemistry of catalase at a gold electrode modified with single-wall carbon nanotubes
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Direct electrochemistry of catalase at a gold electrode modified with single-wall carbon nanotubes

机译：在单壁碳纳米管修饰的金电极上过氧化氢酶的直接电化学

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The direct electrochemistry of catalase (Ct) was accomplished at a gold electrode modified with single-wall carbon nanotubes (SWNTs). A pair of well-defined redox peaks was obtained for Ct with the reduction peak potential at -0.414 V and a peak potential separation of 32 mV at pH 5.9. Both reflectance FT-IR spectra and. the dependence of the reduction peak current on the scan rate revealed that Ct adsorbed onto the SWNT surfaces. The redox wave corresponds to the Fe(III)/Fe(II) redox center of the heme group of the Ct adsorbate. Compared to other types of carbonaccous electrode materials (e.g., graphite and carbon soot), the electron transfer rate of Ct redox reaction was greatly enhanced at the SWNT-modified electrode. The peak current was found to increase linearly with the Ct concentration in the range of 8 x 10(-6)-8 x 10(-5) M Used for the electrode preparation and the peak potential was shown to be pH dependent. The catalytic activity of Ct adsorbates at the SWNTs appears to be retained, as the addition of H2O2 produced a characteristic catalytic redox wave. This work demonstrates that direct electrochemistry of redox-active biomacromolecules such as metalloenzymes can be improved through the use of carbon nanotubes.
机译：过氧化氢酶（Ct）的直接电化学是在用单壁碳纳米管（SWNTs）修饰的金电极上完成的。获得了一对明确定义的Ct氧化还原峰，其还原峰电势为-0.414 V，在pH 5.9时峰电势分离为32 mV。既有反射红外光谱又有。还原峰值电流对扫描速率的依赖性表明，Ct吸附在SWNT表面上。氧化还原波对应于Ct吸附物血红素基团的Fe（III）/ Fe（II）氧化还原中心。与其他类型的含碳电极材料（例如石墨和碳黑）相比，SWNT修饰电极大大提高了Ct氧化还原反应的电子传递速率。发现峰值电流随Ct浓度在8 x 10（-6）-8 x 10（-5）M范围内线性增加，用于电极制备，并且峰值电势与pH有关。 Ct吸附物在SWNT处的催化活性似乎得以保留，因为加入H2O2会产生特征性的催化氧化还原波。这项工作表明，可以通过使用碳纳米管来改善氧化还原活性生物大分子（如金属酶）的直接电化学。

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