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MicroITIES detection of adenosine phosphates

机译:微生物检测磷酸腺苷

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摘要

The complexation between a triamide ligand derived from tris-2-(aminoethyl)amine: N-(2-[bis-(2-(4-tert-butylbenzoyl) -aminoethyl)-amino]-ethyl) 4-tert-butylbenzamide, L, and the three adenosine-containing nucleotides, ATP, ADP, and AMP, was investigated by facilitated ion transfer processes through a microhole array film. Differential pulse voltammetry (DPV) was used to measure the transfer currents for the respective nucleotides. The three nucleotides were found to have different transfer potentials with transfer currents proportional to their aqueous concentrations. Based on the differences of the transfer potentials, it is concluded that the host ligand, L, interacts with the phosphate moiety and the Gibbs transfer energy is dominated by the charge generated by the phosphate groups. The linear relationship between the current response and nucleotide concentration forms the basis of an anion sensor with a dynamic range from 0.1 mm to 5 mm.
机译:源自三-2-(氨基乙基)胺的三酰胺配体之间的络合:N-(2- [双-(2-(2-(4-叔丁基苯甲酰基)-氨基乙基)-氨基]-乙基)4-叔丁基苯甲酰胺,通过微孔阵列膜中的离子转移过程,研究了L和三个含腺苷的核苷酸ATP,ADP和AMP。使用差分脉冲伏安法(DPV)来测量各个核苷酸的转移电流。发现这三个核苷酸具有不同的转移电位,且转移电流与它们的含水浓度成正比。基于转移电势的差异,可以得出结论,主体配体L与磷酸盐部分相互作用,吉布斯转移能主要由磷酸盐基团产生的电荷控制。电流响应和核苷酸浓度之间的线性关系构成了阴离子传感器的基础,其动态范围为0.1 mm至5 mm。

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