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Photoactivity characteristics of a biodevice using primary photosynthetic reaction centers

机译:使用初级光合作用反应中心的生物设备的光活性特征

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Films with alternating layers of thylakoid membrane from the cyanobacterium Spirulina platensis and the positively charged polyion poly(ethylenimine) (PEI) were prepared by means of alternate electrostatic layer-by-layer assembly. The thylakoid membrane/PEI bilayers were functionally and structurally characterized by visible spectra, electrochemical methods and SEM techniques, respectively. The films deposited onto gold electrodes were molecularly smooth and consistent with thylakoid membrane/PEI bilayers as observed by SEM. The photoactivity of the thylakoid membrane/PEI assembly was dependent on thickness of the film that increased with the number of bilayers. There was an obvious difference in the current responses of 1- and 5-bilayer thylakoid membrane/PEI films under illumination. The photosynthetic electron transfer process in the thylakoid membrane/PEI single-bilayer film was demonstrated by an inhibition of the photoactivity by herbicide. Biodevices for the detection of phytotoxicity were constructed using the isolated thylakoid membrane from chloroplast as biosensing elements by means of electrostatic layer-by-layer assembly.
机译:通过交替的静电逐层组装,制备了蓝藻螺旋藻和带正电的聚离子聚(乙烯亚胺)(PEI)的类囊体膜交替层的薄膜。类囊体膜/ PEI双层分别通过可见光谱,电化学方法和SEM技术进行功能和结构表征。 SEM观察到,沉积在金电极上的膜分子光滑且与类囊体膜/ PEI双层一致。类囊体膜/ PEI组件的光活性取决于膜的厚度,该膜的厚度随双层数的增加而增加。 1和5双层类囊体膜/ PEI膜在光照下的电流响应存在明显差异。类囊体膜/ PEI单双层膜中的光合作用电子转移过程通过除草剂抑制光活性来证明。利用从叶绿体分离的类囊体膜作为生物传感元件,构建了用于检测植物毒性的生物装置,并通过静电逐层组装的方式进行了构建。

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