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首页> 外文期刊>Electroanalysis >Amperometric detection of catecholamine neurotransmitters using electrocatalytic substrate recycling at a laccase electrode
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Amperometric detection of catecholamine neurotransmitters using electrocatalytic substrate recycling at a laccase electrode

机译：在漆酶电极上使用电催化底物循环利用电流法检测儿茶酚胺神经递质

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摘要

An enzyme electrode based on the coirnmobilization of an osmium redox polymer and laccase on glassy carbon electrodes has been applied to ultra sensitive amperometric detection of the catecholamine neurotransmitters dopamine, epinephrine and norepinephrine, resulting in nanomolar detection limits, as low as 4 nM for dopamine. The sensitivity of the electrode is due to signal amplification via oxidation of the catecholamine by the immobilized laccase, which is regenerated by concomitant reduction of oxygen to water, coupled to the electrocatalytic rereduction of the oxidized catecholamine by the osmium redox complex: electrocatalytic substrate recycling. In addition because the sensor can be operated in reductive mode at - 0.2 V (vs. Ag/AgCI), noise and interferences are diminished. Combined with its high sensitivity this enzyme electrode also exhibited excellent selectivity allowing the detection of catecholamines in the presence of ascorbic acid. However, differentiation between the current responses achieved for the three catecholarnines is not possible. The effective mode of constant recycling, resulting in amplification of the current response, of the laccase enzyme electrode sensor combined with the inherent advantages of using electrochemical techniques holds great promise for the future of catecholamine detection and monitoring.
机译：基于an氧化还原聚合物和漆酶在玻璃碳电极上的共迁移的酶电极已被用于儿茶酚胺神经递质多巴胺，肾上腺素和去甲肾上腺素的超灵敏安培检测，导致多巴胺的纳摩尔检测限低至4 nM。电极的敏感性归因于固定化漆酶氧化儿茶酚胺而产生的信号放大，该漆酶通过将氧气同时还原为水而再生，再加上氧化还原络合物对氧化的儿茶酚胺的电催化还原作用：电催化底物的回收利用。另外，由于传感器可以在-0.2 V（vs. Ag / AgCI）的还原模式下运行，因此可以减少噪声和干扰。结合其高灵敏度，该酶电极还表现出出色的选择性，可在抗坏血酸存在下检测儿茶酚胺。但是，不可能区分三种儿茶酚胺的当前反应。漆酶酶电极传感器不断循环的有效模式，导致电流响应的放大，结合使用电化学技术的固有优势，为儿茶酚胺检测和监测的未来提供了广阔前景。

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